Azon publikációk listája, ahol a HUMAC s.r.o. termékeit használták:
1.
Zigo, František; Šamudovská, Alena Hreško; Bujňák, Lukáš
Carcass Characteristic, Caecal Metabolites and Dropping Quality in Broiler Chickens Fed Diets Containing a Humic Substances Journal Article
In: Asian Journal of Agriculture and Food Sciences, vol. 10, iss. 6, pp. 133-138, 2023.
@article{nokey,
title = {Carcass Characteristic, Caecal Metabolites and Dropping Quality in Broiler Chickens Fed Diets Containing a Humic Substances},
author = {František Zigo and Alena Hreško Šamudovská and Lukáš Bujňák},
doi = {10.24203/ajafs.v10i6.7116},
year = {2023},
date = {2023-01-01},
journal = {Asian Journal of Agriculture and Food Sciences},
volume = {10},
issue = {6},
pages = {133-138},
abstract = {The objective of the study was to determine the effect of humic substances added to diets on carcass characteristics, weight of visceral organs, fermentation process in the caecum and excretion of dry matter and crude protein in broiler chicks. One hundred one day old broiler chicks were assigned in two equal groups. Birds of the experimental group were fed with diets supplemented with humic substances (in amount: 5 g.kg-1 during the first two weeks and 7 g.kg-1 from the 3rd to the 6th week). No significant effect of humic substances supplementation was observed on carcass characteristics (carcass weight, carcass yield, abdominal fat pad) as well as the relative weight of the liver, the heart, the spleen and the bursa of Fabricius. On the other hand, in the experimental group was found significantly higher relative weight of the pancreas (P < 0.05). Also, the concentration of short-chain fatty acids in the caecum content was not significantly affected, but tested substance lowered the pH value in caecum (P < 0.05). Significantly lover content of crude protein (P < 0.05) and also significantly higher concentration of dry matter (P < 0.01) in the droppings of chicks was found in experimental group than in the control group. It was concluded that the addition of humic substances to the diets can contribute to reducing the burden on the environment from poultry farms.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The objective of the study was to determine the effect of humic substances added to diets on carcass characteristics, weight of visceral organs, fermentation process in the caecum and excretion of dry matter and crude protein in broiler chicks. One hundred one day old broiler chicks were assigned in two equal groups. Birds of the experimental group were fed with diets supplemented with humic substances (in amount: 5 g.kg-1 during the first two weeks and 7 g.kg-1 from the 3rd to the 6th week). No significant effect of humic substances supplementation was observed on carcass characteristics (carcass weight, carcass yield, abdominal fat pad) as well as the relative weight of the liver, the heart, the spleen and the bursa of Fabricius. On the other hand, in the experimental group was found significantly higher relative weight of the pancreas (P < 0.05). Also, the concentration of short-chain fatty acids in the caecum content was not significantly affected, but tested substance lowered the pH value in caecum (P < 0.05). Significantly lover content of crude protein (P < 0.05) and also significantly higher concentration of dry matter (P < 0.01) in the droppings of chicks was found in experimental group than in the control group. It was concluded that the addition of humic substances to the diets can contribute to reducing the burden on the environment from poultry farms.
2.
Skalická, Magdaléna; Naď, Pavel; Bujňák, Lukáš; Marcin, Andrej
The Effect of Humic Substances on the Content of Copper and Zinc in the Turkey Muscle Journal Article
In: Asian Journal of Agriculture and Food Sciences, vol. 10, iss. 6, pp. 108-112, 2023.
@article{nokey,
title = {The Effect of Humic Substances on the Content of Copper and Zinc in the Turkey Muscle},
author = {Magdaléna Skalická and Pavel Naď and Lukáš Bujňák and Andrej Marcin},
doi = {10.24203/ajafs.v10i6.7099},
year = {2023},
date = {2023-01-01},
journal = {Asian Journal of Agriculture and Food Sciences},
volume = {10},
issue = {6},
pages = {108-112},
abstract = {The aim of the study was to assess the effect of humic substances on the changes of copper (Cu) and zinc (Zn) content in the thigh and breast muscle of turkey. For the trial, 20 turkeys (BIG 6) were allocated into two groups (control group and experimental group) of 10 birds. Experimental group was supplemented with 0.5 % Humac Mycotoxi Sorb (HM). The control group received a basal diet without any supplements. The Humac Mycotoxi Sorb was added to turkey feed for 10 weeks. In the breast and thigh muscles, we found a statistically significantly higher (P < 0.001) Cu content after the addition of 0.5% HM to the feed (4.43; 4.18 mg.kg-1 ) compared to the control group of turkeys (2.70;3.35 mg.kg-1). Also, in the breast and thigh muscles, were found a statistically significantly higher (P < 0.05) Zn content after the addition of 0.5% HM to the feed (50.98; 64.42 mg.kg-1 ) compared to the control group of turkeys (44.33; 62.95 mg.kg-1).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of the study was to assess the effect of humic substances on the changes of copper (Cu) and zinc (Zn) content in the thigh and breast muscle of turkey. For the trial, 20 turkeys (BIG 6) were allocated into two groups (control group and experimental group) of 10 birds. Experimental group was supplemented with 0.5 % Humac Mycotoxi Sorb (HM). The control group received a basal diet without any supplements. The Humac Mycotoxi Sorb was added to turkey feed for 10 weeks. In the breast and thigh muscles, we found a statistically significantly higher (P < 0.001) Cu content after the addition of 0.5% HM to the feed (4.43; 4.18 mg.kg-1 ) compared to the control group of turkeys (2.70;3.35 mg.kg-1). Also, in the breast and thigh muscles, were found a statistically significantly higher (P < 0.05) Zn content after the addition of 0.5% HM to the feed (50.98; 64.42 mg.kg-1 ) compared to the control group of turkeys (44.33; 62.95 mg.kg-1).
3.
Tóth, Štefan; Šoltysová, Božena; Porvaz, Pavol
Soil Reaction and Sugar Beet (Influence of Soil Liming and Rapid Effectiveness of Composite Humino-Calcium Soil Preparations on Soil pH Change) Journal Article
In: Listy cukrovarnické a řepařské, no. 139, 2023, ISSN: 1805-9708.
@article{nokey,
title = {Soil Reaction and Sugar Beet (Influence of Soil Liming and Rapid Effectiveness of Composite Humino-Calcium Soil Preparations on Soil pH Change)},
author = {Štefan Tóth and Božena Šoltysová and Pavol Porvaz},
issn = {1805-9708},
year = {2023},
date = {2023-01-01},
urldate = {2023-01-01},
journal = {Listy cukrovarnické a řepařské},
number = {139},
abstract = {The presented work focuses on summarizing the effect of soil
reaction on sugar beet stands, including the principles of soil liming,
and also on summarizing the effectiveness of composite humiccalcined
soil preparations on soil pH change.
The efficacy of the composite preparations was tested in a polyfactorial
ex-situ experiment, based on four soil types and using a remote
range with dose variability. Three soil preparations were tested, of
which 2 humic-calcium and 1 soil conditioner based on humic acids.
The calcium component of the composite preparations consists of
chalk, the natural equivalent of limestone characterized by a high
degree of purity and a calcium carbonate content of up to 90%.
Humic acids, a natural stimulator of soil fertility, are an additive
mixed in an amount of 10% or 30%. The results of the experiment,
which lasted 30 days under standard conditions, confirm the high
effectiveness of those preparations in changing the soil pH.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The presented work focuses on summarizing the effect of soil
reaction on sugar beet stands, including the principles of soil liming,
and also on summarizing the effectiveness of composite humiccalcined
soil preparations on soil pH change.
The efficacy of the composite preparations was tested in a polyfactorial
ex-situ experiment, based on four soil types and using a remote
range with dose variability. Three soil preparations were tested, of
which 2 humic-calcium and 1 soil conditioner based on humic acids.
The calcium component of the composite preparations consists of
chalk, the natural equivalent of limestone characterized by a high
degree of purity and a calcium carbonate content of up to 90%.
Humic acids, a natural stimulator of soil fertility, are an additive
mixed in an amount of 10% or 30%. The results of the experiment,
which lasted 30 days under standard conditions, confirm the high
effectiveness of those preparations in changing the soil pH.
reaction on sugar beet stands, including the principles of soil liming,
and also on summarizing the effectiveness of composite humiccalcined
soil preparations on soil pH change.
The efficacy of the composite preparations was tested in a polyfactorial
ex-situ experiment, based on four soil types and using a remote
range with dose variability. Three soil preparations were tested, of
which 2 humic-calcium and 1 soil conditioner based on humic acids.
The calcium component of the composite preparations consists of
chalk, the natural equivalent of limestone characterized by a high
degree of purity and a calcium carbonate content of up to 90%.
Humic acids, a natural stimulator of soil fertility, are an additive
mixed in an amount of 10% or 30%. The results of the experiment,
which lasted 30 days under standard conditions, confirm the high
effectiveness of those preparations in changing the soil pH.
4.
Kovacik, Anton; Sladecek, Tomas; Jurcik, Rastislav; Massanyi, Martin; Kovacikova, Eva; Jambor, Tomas; Hleba, Lukas; Cubon, Juraj; Vizzarri, Francesco; Massanyi, Peter
Impact of Humic Acids in Nutritionon Haematological and Biochemical Parametersof Brown Hares Journal Article
In: Journal of Microbiology, Biotechnology and Food Sciences, 2022, ISSN: 1338-5178.
@article{nokey,
title = {Impact of Humic Acids in Nutritionon Haematological and Biochemical Parametersof Brown Hares},
author = {Anton Kovacik and Tomas Sladecek and Rastislav Jurcik and Martin Massanyi and Eva Kovacikova and Tomas Jambor and Lukas Hleba and Juraj Cubon and Francesco Vizzarri and Peter Massanyi},
doi = {10.55251/jmbfs.9549},
issn = {1338-5178},
year = {2022},
date = {2022-10-18},
journal = {Journal of Microbiology, Biotechnology and Food Sciences},
abstract = {Humic substances are natural organic substances resulting from the decomposition of mainly plant but also animal residues. The objective of our study was to test if the humic acids can negatively/positively affect brown hares health status. As the main indicators for this evaluation, we chose haematological and biochemical blood tests. In this study, we used 24 brown hares (12 males and 12 females) in the age of 12-24 months. As part of the 6-month experiment, we planned three blood samplings (at the beginning of the experiment and then after three and six months). Animals were divided into three groups at the beginning of the experiment (n = 8/group): CG (control group, 0-11KKZ standard diet without additives), EG1 group (experimental group 1, received 0-10KK/D standard feed + 1% of Humac Natur AFM – humic acids), and EG2 group (experimental group 2, received 0-10KK/D standard feed + 1% of Humac Natur AFM – humic acids, enriched with green feed – clover-grass mixture). We found a statistically significant difference between the control group and the EG2 group in the RBC parameter (P < 0.05) after three months of consumption. We found a statistically significant decrease in urea levels in both experimental groups compared to the control group (P < 0.001), as well as a decrease in urea concentration in the EG2 group compared to the EG1 group (P < 0.01); decrease in cholesterol levels in the experimental groups compared to the control, and between EG1 and the control group, this decrease was statistically significant (P < 0.05); and we recorded a statistically significant increase of triglycerides in the EG2 group compared to the other groups (P < 0.01). Based on the obtained results, we can evaluate the use of 1% humic acid as a feed additive as safe for feeding hares.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Humic substances are natural organic substances resulting from the decomposition of mainly plant but also animal residues. The objective of our study was to test if the humic acids can negatively/positively affect brown hares health status. As the main indicators for this evaluation, we chose haematological and biochemical blood tests. In this study, we used 24 brown hares (12 males and 12 females) in the age of 12-24 months. As part of the 6-month experiment, we planned three blood samplings (at the beginning of the experiment and then after three and six months). Animals were divided into three groups at the beginning of the experiment (n = 8/group): CG (control group, 0-11KKZ standard diet without additives), EG1 group (experimental group 1, received 0-10KK/D standard feed + 1% of Humac Natur AFM – humic acids), and EG2 group (experimental group 2, received 0-10KK/D standard feed + 1% of Humac Natur AFM – humic acids, enriched with green feed – clover-grass mixture). We found a statistically significant difference between the control group and the EG2 group in the RBC parameter (P < 0.05) after three months of consumption. We found a statistically significant decrease in urea levels in both experimental groups compared to the control group (P < 0.001), as well as a decrease in urea concentration in the EG2 group compared to the EG1 group (P < 0.01); decrease in cholesterol levels in the experimental groups compared to the control, and between EG1 and the control group, this decrease was statistically significant (P < 0.05); and we recorded a statistically significant increase of triglycerides in the EG2 group compared to the other groups (P < 0.01). Based on the obtained results, we can evaluate the use of 1% humic acid as a feed additive as safe for feeding hares.
5.
Kwiatkowski, Cezary A.; Harasim, Elżbieta; Feledyn-Szewczyk, Beata; Stalenga, Jarosław; Jańczak-Pieniążek, Marta; Buczek, Jan; Nnolim, Agnieszka
In: Agriculture, vol. 12, iss. 10, pp. 1534, 2022.
@article{nokey,
title = {Productivity and Quality of Chamomile (Chamomilla recutita (L.) Rausch.) Grown in an Organic System Depending on Foliar Biopreparations and Row Spacing},
author = {Cezary A. Kwiatkowski and Elżbieta Harasim and Beata Feledyn-Szewczyk and Jarosław Stalenga and Marta Jańczak-Pieniążek and Jan Buczek and Agnieszka Nnolim},
editor = {Daniele Del Buono},
doi = {10.3390/agriculture12101534},
year = {2022},
date = {2022-09-23},
journal = {Agriculture},
volume = {12},
issue = {10},
pages = {1534},
abstract = {The study involved a field experiment conducted on two cultivars of chamomile (‘Złoty Łan’ and ‘Mastar’) in the climatic and soil conditions of the central Lublin region (Poland) during the years 2016–2018. The experiment was designed to determine the effects of three foliar biological preparations (growth stimulant Bio-algeen, fertilizer Herbagreen Basic, and Effective Microorganisms applied as EM Farming spray), which were applied once or twice, on the yield and quality of herbal raw material of chamomile grown under organic conditions. Chamomile was grown at different row spacings (40 cm and 30 cm). The biopreparations (in particular Herbagreen Basic) had a positive effect on chamomile yield (about 10–11% in comparison with control treatment) and yield attributing characters (plant height, number of branches, and inflorescences per plant) as well as on the quality parameters. The EM Farming had a minimal influence on the quantity characteristics studied, but it beneficially affected essential oil and chlorophyll content. The biopreparations had a more favorable effect when they were applied twice. The wider row spacing of chamomile (40 cm) promoted higher yields (about 18%) compared to 30 cm. The narrower spacing (30 cm), however, contributed to better quality characteristics of herbal raw material. The study confirmed much higher productivity and quality of the cultivar ‘Złoty Łan’ compared to cv. ‘Mastar’ (about 15%). Significant interactions of experimental factors concerned mainly the formation of the essential oil content in herbal raw material (the most advantageous was the ‘Złoty Łan’ cultivar sown at a row spacing of 30 cm with the use of Herbagreen Basic biopreparation twice).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The study involved a field experiment conducted on two cultivars of chamomile (‘Złoty Łan’ and ‘Mastar’) in the climatic and soil conditions of the central Lublin region (Poland) during the years 2016–2018. The experiment was designed to determine the effects of three foliar biological preparations (growth stimulant Bio-algeen, fertilizer Herbagreen Basic, and Effective Microorganisms applied as EM Farming spray), which were applied once or twice, on the yield and quality of herbal raw material of chamomile grown under organic conditions. Chamomile was grown at different row spacings (40 cm and 30 cm). The biopreparations (in particular Herbagreen Basic) had a positive effect on chamomile yield (about 10–11% in comparison with control treatment) and yield attributing characters (plant height, number of branches, and inflorescences per plant) as well as on the quality parameters. The EM Farming had a minimal influence on the quantity characteristics studied, but it beneficially affected essential oil and chlorophyll content. The biopreparations had a more favorable effect when they were applied twice. The wider row spacing of chamomile (40 cm) promoted higher yields (about 18%) compared to 30 cm. The narrower spacing (30 cm), however, contributed to better quality characteristics of herbal raw material. The study confirmed much higher productivity and quality of the cultivar ‘Złoty Łan’ compared to cv. ‘Mastar’ (about 15%). Significant interactions of experimental factors concerned mainly the formation of the essential oil content in herbal raw material (the most advantageous was the ‘Złoty Łan’ cultivar sown at a row spacing of 30 cm with the use of Herbagreen Basic biopreparation twice).
6.
Kwiatkowski, Cezary A.; Harasim, Elżbieta; Feledyn-Szewczyk, Beata; Joniec, Jolanta
Open AccessArticle The Antioxidant Potential of Grains in Selected Cereals Grown in an Organic and Conventional System Journal Article
In: Agriculture, vol. 12, iss. 9, pp. 1485, 2022.
@article{nokey,
title = { Open AccessArticle The Antioxidant Potential of Grains in Selected Cereals Grown in an Organic and Conventional System},
author = {Cezary A. Kwiatkowski and Elżbieta Harasim and Beata Feledyn-Szewczyk and Jolanta Joniec},
editor = {Ilaria Marotti},
doi = {10.3390/agriculture12091485},
year = {2022},
date = {2022-09-16},
journal = {Agriculture},
volume = {12},
issue = {9},
pages = {1485},
abstract = {The paper presents the effect of conventional (use of NPK mineral fertilizers and pesticides) and organic (no use of agrochemicals) farming systems on selected parameters of antioxidant properties of winter wheat, spring barley and oat grain. The research was carried out during the period 2017–2019 at the Czesławice Experimental Farm (central Lublin region, Poland) on loess soil (second quality class). The aim of the research was to evaluate the functional (antioxidant) properties of winter wheat, spring barley and oat grain in whole grain and its milling fractions (dehulled grain, flour and bran). The reduction potential (Fe+3 → Fe+2 ), the ability to eliminate the free DPPH• radical and the total antioxidant potential in the β-carotene/linoleic acid system were determined. Polyphenol content was also determined using Folin–Ciocalteau reagent. The organic system did not significantly increase the antioxidant properties of cereal grains compared to the conventional system. Under organic farming conditions, oat grain was characterised only by the most favourable antioxidant properties. A highly statistically significant correlation was found between total polyphenol content and DPPH• free radical quenching capacity, especially for oat and barley in the organic system. The closest correlations were for the fractions of bran and whole grain. Dehulling of grain, with the exception of oat grain, irrespective of the farming system, resulted in a significant deterioration of the antioxidant potential of grain extracts. In summary, the study showed that the bran obtained from oat grown under an organic system had the strongest antioxidant activity.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The paper presents the effect of conventional (use of NPK mineral fertilizers and pesticides) and organic (no use of agrochemicals) farming systems on selected parameters of antioxidant properties of winter wheat, spring barley and oat grain. The research was carried out during the period 2017–2019 at the Czesławice Experimental Farm (central Lublin region, Poland) on loess soil (second quality class). The aim of the research was to evaluate the functional (antioxidant) properties of winter wheat, spring barley and oat grain in whole grain and its milling fractions (dehulled grain, flour and bran). The reduction potential (Fe+3 → Fe+2 ), the ability to eliminate the free DPPH• radical and the total antioxidant potential in the β-carotene/linoleic acid system were determined. Polyphenol content was also determined using Folin–Ciocalteau reagent. The organic system did not significantly increase the antioxidant properties of cereal grains compared to the conventional system. Under organic farming conditions, oat grain was characterised only by the most favourable antioxidant properties. A highly statistically significant correlation was found between total polyphenol content and DPPH• free radical quenching capacity, especially for oat and barley in the organic system. The closest correlations were for the fractions of bran and whole grain. Dehulling of grain, with the exception of oat grain, irrespective of the farming system, resulted in a significant deterioration of the antioxidant potential of grain extracts. In summary, the study showed that the bran obtained from oat grown under an organic system had the strongest antioxidant activity.
7.
Lacková, Zuzana; Zigo, František; Farkašová, Zuzana; Ondrašovičová, Silvia
In: Life, vol. 12, no. 7, pp. 1016, 2022, ISSN: 2075-1729.
@article{nokey,
title = {The Effect of Humic Substances as an Organic Supplement on the Fattening Performance, Quality of Meat, and Selected Biochemical Parameters of Rabbits},
author = {Zuzana Lacková and František Zigo and Zuzana Farkašová and Silvia Ondrašovičová},
doi = {10.3390/life12071016},
issn = {2075-1729},
year = {2022},
date = {2022-07-08},
urldate = {2022-07-08},
journal = {Life},
volume = {12},
number = {7},
pages = {1016},
abstract = {In this study, we assessed the effect of humic substances, as an organic supplement in feed, on the fattening performance, meat quality and selected lipid and mineral parameters from the blood serum of rabbits. Three groups of the Giant Saris rabbit breed were used (one control and two experimental), with 16 animals per group. The animals in the control group were fed a standard pellet diet, the humic substances group received a basal diet supplemented with 5% humic substances, and the third group received a basal diet with 5% humic–fatty substances preparation during the entire experiment (from 35 to 120 days of age). There were 85 days of fattening; then, the rabbits were slaughtered. In the group supplemented with 5% humic–fatty substances addition, we noticed a higher final weight (p < 0.05) and higher average daily gains compared to the control group at the end of the fattening period, at 120 days of age. On the other hand, a slightly lower final weight (p > 0.05) in the group supplemented with humic substances was found compared to the control group. In the comparison of the individual parameters of the meat quality in rabbits, we observed a positive effect in the reduction in the intramuscular fat content and the lipid parameters as well as a lower total cholesterol from the blood serum in both supplemented groups. Regarding the mineral parameters, we observed elevated blood serum values of calcium and phosphorus in both experimental groups. The addition of humic–fatty substances appears to be the most effective way of supplementing rabbit feed due to the synergistic effect of humates and vegetable oils for their optimal growth development and the production of reduced-fat meat.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this study, we assessed the effect of humic substances, as an organic supplement in feed, on the fattening performance, meat quality and selected lipid and mineral parameters from the blood serum of rabbits. Three groups of the Giant Saris rabbit breed were used (one control and two experimental), with 16 animals per group. The animals in the control group were fed a standard pellet diet, the humic substances group received a basal diet supplemented with 5% humic substances, and the third group received a basal diet with 5% humic–fatty substances preparation during the entire experiment (from 35 to 120 days of age). There were 85 days of fattening; then, the rabbits were slaughtered. In the group supplemented with 5% humic–fatty substances addition, we noticed a higher final weight (p < 0.05) and higher average daily gains compared to the control group at the end of the fattening period, at 120 days of age. On the other hand, a slightly lower final weight (p > 0.05) in the group supplemented with humic substances was found compared to the control group. In the comparison of the individual parameters of the meat quality in rabbits, we observed a positive effect in the reduction in the intramuscular fat content and the lipid parameters as well as a lower total cholesterol from the blood serum in both supplemented groups. Regarding the mineral parameters, we observed elevated blood serum values of calcium and phosphorus in both experimental groups. The addition of humic–fatty substances appears to be the most effective way of supplementing rabbit feed due to the synergistic effect of humates and vegetable oils for their optimal growth development and the production of reduced-fat meat.
8.
Holátko, Jiří; Hammerschmiedt, Tereza; Latal, Oldřich; Kintl, Antonin; Mustafa, Adnan; Baltazar, Tivadar; Malíček, Ondrej; Brtnický, Martin
Deciphering the Effectiveness of Humic Substances and Biochar Modified Digestates on Soil Quality and Plant Biomass Accumulation Journal Article
In: Agronomy, vol. 12, iss. 7, pp. 1587, 2022.
@article{nokey,
title = {Deciphering the Effectiveness of Humic Substances and Biochar Modified Digestates on Soil Quality and Plant Biomass Accumulation},
author = {Jiří Holátko and Tereza Hammerschmiedt and Oldřich Latal and Antonin Kintl and Adnan Mustafa and Tivadar Baltazar and Ondrej Malíček and Martin Brtnický},
doi = {10.3390/agronomy12071587},
year = {2022},
date = {2022-06-30},
journal = {Agronomy},
volume = {12},
issue = {7},
pages = {1587},
abstract = {The effective use of digestate as exogenous organic matter to enhance soil carbon sequestration depends on the balance between labile and recalcitrant organic carbon, which is influenced by the type of feedstock, the fermentation process, and the fraction (liquid, solid) of the fermented product used. In this work, in order to change the ratio of labile to stable carbon in the resulting fertiliser, the digestate was mixed with organic carbon-rich supplements: biochar and Humac (a humic acid-rich substance). The pot experiment was carried out under controlled conditions with maize (Zea mays L.) in soil amended with the digestate (D), which was incubated with Humac (H), biochar (B), or a combination of both (D + B + H) before the application. Digestate enriched with Humac showed improved short-term nutrient (carbon, phosphorus, nitrogen) transformation, as indicated by soil enzyme activity and the highest maize biomass production of. Total carbon content, C:N ratio, short-term respiration activity, and nitrification were most enhanced by digestate enriched with either biochar or combined biochar + Humac). Long-term nitrogen mineralization was mostly enhanced by digestate + Humac, as indicated by amino-acid-induced respiration and urease activity. Short-term positive effects of digestate + biochar (eventually + Humac) on catabolism were proven, whereas their long-term effects on nutrient mineralization were negative (i.e., biochar-mediated immobilization, sequestration), which should be the focus of further research in future.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effective use of digestate as exogenous organic matter to enhance soil carbon sequestration depends on the balance between labile and recalcitrant organic carbon, which is influenced by the type of feedstock, the fermentation process, and the fraction (liquid, solid) of the fermented product used. In this work, in order to change the ratio of labile to stable carbon in the resulting fertiliser, the digestate was mixed with organic carbon-rich supplements: biochar and Humac (a humic acid-rich substance). The pot experiment was carried out under controlled conditions with maize (Zea mays L.) in soil amended with the digestate (D), which was incubated with Humac (H), biochar (B), or a combination of both (D + B + H) before the application. Digestate enriched with Humac showed improved short-term nutrient (carbon, phosphorus, nitrogen) transformation, as indicated by soil enzyme activity and the highest maize biomass production of. Total carbon content, C:N ratio, short-term respiration activity, and nitrification were most enhanced by digestate enriched with either biochar or combined biochar + Humac). Long-term nitrogen mineralization was mostly enhanced by digestate + Humac, as indicated by amino-acid-induced respiration and urease activity. Short-term positive effects of digestate + biochar (eventually + Humac) on catabolism were proven, whereas their long-term effects on nutrient mineralization were negative (i.e., biochar-mediated immobilization, sequestration), which should be the focus of further research in future.
9.
Tóth, Štefan; Szanyi, Gejza; Vančo, Peter; Schubert, Ján; Porvaz, Pavol; Bujňák, Peter; Šoltysová, Božena; Danielovič, Igor
In: European Pharmaceutical Journal, vol. 69, no. 1, pp. 27 - 36, 2022, ISSN: 2453-6725.
@article{nokey,
title = {The influence of mineral nutrition and humic acids on the intensity of photosynthesis, as well as the yield and quality of seeds, roots, and aboveground phytomass of milk thistle Silybum marianum (L.) Gaertn. in marginal growing conditions},
author = {Štefan Tóth and Gejza Szanyi and Peter Vančo and Ján Schubert and Pavol Porvaz and Peter Bujňák and Božena Šoltysová and Igor Danielovič},
doi = {10.2478/afpuc-2022-0003},
issn = {2453-6725},
year = {2022},
date = {2022-06-24},
urldate = {2022-06-24},
journal = {European Pharmaceutical Journal},
volume = {69},
number = {1},
pages = {27 - 36},
abstract = {Milk thistle Silybum marianum (L.) Gaertn. is one of the most researched medicinal plants, but at the same time it belongs to crops with wide industry potential whose growth requirements for marginal agroecological conditions or the response to agronomic interventions has not yet been sufficiently studied. The aim of the present research was to verify the effect of mineral nutrition (NPK 200 kg/ha) and humic acids applied to the soil (HUMAC Agro 250 kg/ha) on the main biometric parameters and the intensity of photosynthesis of milk thistle grown under less favourable climatic conditions of the semi-cold and humid foothills in Central Europe. The highest seed yield was achieved with the NPK variant in terms of dry matter 0.534 t/ha, in the HUMAC Agro variant 0.254 t/ha and with the untreated control 0.087 t/ha. The soil before the trial establishment was characterised by low nutrient content. The use of mineral fertiliser on NPK treatment eliminated the influence of low PK nutrients content of the soil, influencing the crop growth and yield/quality, while no doses of NPK were used on the HUMAC Agro variant and the untreated control. For both of the two sequences created (temperature and light), the highest photosynthesis rate was measured on untreated control (20.115 and 12.386 mmol/m2/s1), markedly lower on HUMAC Agro (16.386 and 9.653 mmol/m2/s1) – and the lowest on the NPK (10.933 and 7.813 mmol/m2/s1, respectively), in inverse proportion to the crop yield. Therefore, the size of the leaf area of the crop was decisive for the increased crop yield. The polynomial trend line of the photosynthesis rate according to the temperature sequence shows the photosynthesis rate and the torrential decrease by temperature increasing on untreated control compared with both treated variants. Both of these threats, mineral nutrition and use of humic preparations to soil, although they reduce the photosynthesis rate, have a wide range of complex effects that provide an opportunity to optimise the growth and yield of milk thistle. Ideally, the mineral nutrition and humic preparations should be used in combination with the crop of milk thistle for medicinal and energy purposes.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Milk thistle Silybum marianum (L.) Gaertn. is one of the most researched medicinal plants, but at the same time it belongs to crops with wide industry potential whose growth requirements for marginal agroecological conditions or the response to agronomic interventions has not yet been sufficiently studied. The aim of the present research was to verify the effect of mineral nutrition (NPK 200 kg/ha) and humic acids applied to the soil (HUMAC Agro 250 kg/ha) on the main biometric parameters and the intensity of photosynthesis of milk thistle grown under less favourable climatic conditions of the semi-cold and humid foothills in Central Europe. The highest seed yield was achieved with the NPK variant in terms of dry matter 0.534 t/ha, in the HUMAC Agro variant 0.254 t/ha and with the untreated control 0.087 t/ha. The soil before the trial establishment was characterised by low nutrient content. The use of mineral fertiliser on NPK treatment eliminated the influence of low PK nutrients content of the soil, influencing the crop growth and yield/quality, while no doses of NPK were used on the HUMAC Agro variant and the untreated control. For both of the two sequences created (temperature and light), the highest photosynthesis rate was measured on untreated control (20.115 and 12.386 mmol/m2/s1), markedly lower on HUMAC Agro (16.386 and 9.653 mmol/m2/s1) – and the lowest on the NPK (10.933 and 7.813 mmol/m2/s1, respectively), in inverse proportion to the crop yield. Therefore, the size of the leaf area of the crop was decisive for the increased crop yield. The polynomial trend line of the photosynthesis rate according to the temperature sequence shows the photosynthesis rate and the torrential decrease by temperature increasing on untreated control compared with both treated variants. Both of these threats, mineral nutrition and use of humic preparations to soil, although they reduce the photosynthesis rate, have a wide range of complex effects that provide an opportunity to optimise the growth and yield of milk thistle. Ideally, the mineral nutrition and humic preparations should be used in combination with the crop of milk thistle for medicinal and energy purposes.
10.
Tomczyńska-Mleko, Marta; Kwiatkowski, Cezary Andrzej; Harasim, Elżbieta; nad Stanisław Mleko, Justyna Leśniowska-Nowak; Terpiłowski, Konrad; Pérez-Huertas, Salvador; Klikocka-Wiśniewska, Olimpia
In: Molecules, vol. 27, iss. 12, pp. 3926, 2022.
@article{nokey,
title = {Influence of Farming System and Forecrops of Spring Wheat on Protein Content in the Grain and the Physicochemical Properties of Unsonicated and Sonicated Gluten},
author = {Marta Tomczyńska-Mleko and Cezary Andrzej Kwiatkowski and Elżbieta Harasim and Justyna Leśniowska-Nowak nad Stanisław Mleko and Konrad Terpiłowski and Salvador Pérez-Huertas and Olimpia Klikocka-Wiśniewska},
editor = {Anthony S. Serianni},
doi = {10.3390/molecules27123926},
year = {2022},
date = {2022-06-19},
urldate = {2022-06-19},
journal = {Molecules},
volume = {27},
issue = {12},
pages = {3926},
abstract = {The potential for enhancing the spring wheat protein content by different cultivation strategies was explored. The influence of ultrasound on the surface and rheological properties of wheat-gluten was also studied. Spring wheat was cultivated over the period of 2018–2020 using two farming systems (conventional and organic) and five forecrops (sugar beet, spring barley, red clover, winter wheat, or oat). The obtained gluten was sonicated using the ultrasonic scrubber. For all organically grown wheat, the protein content was higher than for the conventional one. There was no correlation between the rheological properties of gluten and the protein content in the grain. Gluten derived from organically grown wheat was more elastic than those derived from the conventional one. Sonication enhanced the elasticity of gluten. The sonication effect was influenced by the forecrops. The most elastic gluten after sonication was found for organic barley and sugar beet. The lowest values of tan (delta) were noted for conventional wheat and conventional oat. Cultivation in the monoculture gave gluten with a smaller susceptibility to increase elasticity after sonic treatment. Sonication promoted the cross-linking of protein molecules and induced a more hydrophobic character, which was confirmed by an increment in contact angles (CAs). Most of the organically grown wheat samples showed a lower CA than the conventional ones, which indicated a less hydrophobic character. The gluten surface became rougher with the sonication, regardless of the farming system and applied forecrops. Sonication treatment of gluten proteins rearranged the intermolecular linkages, especially disulfide and hydrophobic bonds, leading to changes in their surface morphology.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The potential for enhancing the spring wheat protein content by different cultivation strategies was explored. The influence of ultrasound on the surface and rheological properties of wheat-gluten was also studied. Spring wheat was cultivated over the period of 2018–2020 using two farming systems (conventional and organic) and five forecrops (sugar beet, spring barley, red clover, winter wheat, or oat). The obtained gluten was sonicated using the ultrasonic scrubber. For all organically grown wheat, the protein content was higher than for the conventional one. There was no correlation between the rheological properties of gluten and the protein content in the grain. Gluten derived from organically grown wheat was more elastic than those derived from the conventional one. Sonication enhanced the elasticity of gluten. The sonication effect was influenced by the forecrops. The most elastic gluten after sonication was found for organic barley and sugar beet. The lowest values of tan (delta) were noted for conventional wheat and conventional oat. Cultivation in the monoculture gave gluten with a smaller susceptibility to increase elasticity after sonic treatment. Sonication promoted the cross-linking of protein molecules and induced a more hydrophobic character, which was confirmed by an increment in contact angles (CAs). Most of the organically grown wheat samples showed a lower CA than the conventional ones, which indicated a less hydrophobic character. The gluten surface became rougher with the sonication, regardless of the farming system and applied forecrops. Sonication treatment of gluten proteins rearranged the intermolecular linkages, especially disulfide and hydrophobic bonds, leading to changes in their surface morphology.
11.
Skalická, Magdaléna; Naď, Pavel; Bujňák, Lukáš; Marcin, Andrej
Contents of Selected Elements in the Blood Serum of Broiler Chickens After Supplementation of Humic Substances Journal Article
In: Folia Veterinaria, vol. 66, iss. 2, pp. 46-53, 2022.
@article{nokey,
title = {Contents of Selected Elements in the Blood Serum of Broiler Chickens After Supplementation of Humic Substances},
author = {Magdaléna Skalická and Pavel Naď and Lukáš Bujňák and Andrej Marcin},
doi = {10.2478/fv-2022-0017},
year = {2022},
date = {2022-05-26},
journal = {Folia Veterinaria},
volume = {66},
issue = {2},
pages = {46-53},
abstract = {This study was conducted to determine the concentrations of mineral substances, such as: calcium (Ca), magnesium (Mg), copper (Cu), and zinc (Zn); in the blood serum of broiler chicks after supplementation of humic substances. Group 1 (G1) was supplemented with 0.7 % Humac Natur Mycosorb (HNMy); G2 with 0.7 % Humac Natur Monogastric (HNM); G3 with 0.3 % HNMy; and the control group (GC) received a basal diet without any supplements. In the G2 group, the serum Ca level increased slightly compared to the other experimental groups. A statistically significant decrease (P < 0.001) in the blood serum of Mg was detected in the broilers from group G2 (0.60 mmol.l ⁻¹ ) and G1 (P < 0.05; 0.68 mmol.l ⁻¹ ) in comparison to the GC. The Zn in the blood serum of broilers from group G2 (22.05 µmol.l ⁻¹ ) was significantly increased (P < 0.05) in broilers from group G2 in comparison to the control group (19.47 µmol.l ⁻¹ ) and G1 group (19.61 µmol.l ⁻¹ ). The serum Cu (12.72 µmol.l ⁻¹ ) was significantly increased (P < 0.001) in broilers from group G2 in comparison to the GC (10.28 µmol.l ⁻¹ ). In the group G1 there was a significant decrease (P < 0.05) in the level of the Cu in the blood serum (8.69 µmol.l ⁻¹ ) in comparison to the serum Cu in broilers from groups G3 (10.45 µmol.l ⁻¹ ) and G2 (12.72 µmol.l ⁻¹ ). The addition of Humac Natur Mycosorb (0.3 % and 0.7 %) in the feed caused a strong correlation in the blood serum between the Ca and Mg (r = 0.7494; r = 0.7648). The addition of 0.3 % Humac Natur Mycosorb in the feed caused a high negative correlation (r = –0.7078) in the blood serum between the Mg and Zn.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study was conducted to determine the concentrations of mineral substances, such as: calcium (Ca), magnesium (Mg), copper (Cu), and zinc (Zn); in the blood serum of broiler chicks after supplementation of humic substances. Group 1 (G1) was supplemented with 0.7 % Humac Natur Mycosorb (HNMy); G2 with 0.7 % Humac Natur Monogastric (HNM); G3 with 0.3 % HNMy; and the control group (GC) received a basal diet without any supplements. In the G2 group, the serum Ca level increased slightly compared to the other experimental groups. A statistically significant decrease (P < 0.001) in the blood serum of Mg was detected in the broilers from group G2 (0.60 mmol.l ⁻¹ ) and G1 (P < 0.05; 0.68 mmol.l ⁻¹ ) in comparison to the GC. The Zn in the blood serum of broilers from group G2 (22.05 µmol.l ⁻¹ ) was significantly increased (P < 0.05) in broilers from group G2 in comparison to the control group (19.47 µmol.l ⁻¹ ) and G1 group (19.61 µmol.l ⁻¹ ). The serum Cu (12.72 µmol.l ⁻¹ ) was significantly increased (P < 0.001) in broilers from group G2 in comparison to the GC (10.28 µmol.l ⁻¹ ). In the group G1 there was a significant decrease (P < 0.05) in the level of the Cu in the blood serum (8.69 µmol.l ⁻¹ ) in comparison to the serum Cu in broilers from groups G3 (10.45 µmol.l ⁻¹ ) and G2 (12.72 µmol.l ⁻¹ ). The addition of Humac Natur Mycosorb (0.3 % and 0.7 %) in the feed caused a strong correlation in the blood serum between the Ca and Mg (r = 0.7494; r = 0.7648). The addition of 0.3 % Humac Natur Mycosorb in the feed caused a high negative correlation (r = –0.7078) in the blood serum between the Mg and Zn.
12.
Buzogáňová, Liliána; Kello, Martin; Bago, Martina; Ogurčáková, Daniela; Vašková, Janka
Assessment of the effects of Annona muricata leaf aqueous extract in vitro Journal Article
In: Emirates Journal of Food and Agriculture, vol. 33, iss. 11, pp. 909-916, 2022.
@article{article,
title = {Assessment of the effects of Annona muricata leaf aqueous extract in vitro},
author = {Liliána Buzogáňová and Martin Kello and Martina Bago and Daniela Ogurčáková and Janka Vašková},
doi = {10.9755/ejfa.2021.v33.i11.2785},
year = {2022},
date = {2022-01-01},
urldate = {2022-01-01},
journal = {Emirates Journal of Food and Agriculture},
volume = {33},
issue = {11},
pages = {909-916},
abstract = {Background: The extracts of Annona muricata, also known as graviola, form part of traditional medicine. Objectives: Verification of their effects and elucidation of their mechanisms is beneficial in terms of the utility of these extracts in treatment or prevention. Methods: We monitored the effectiveness of an extract of dried graviola leaves available for direct consumption on cancer cell lines and isolated rat liver mitochondria. Results: The Jurkat cell line exhibited the highest, sensitivity, with the metabolic activity of cells reaching less than 10% at the highest tested concentration. The effect was dose-dependent. The viability of Jurkat cells was affected after more than the 72 hours. At the same time, there was also a reduction of mitochondrial membrane potential. At 24 to 48 hours incubation, no increase was found in the activity of caspase-3 but increased levels were found for the non-phosphorylated form of Bcl-2. Higher concentrations of graviola affected the mitochondrial redox state by dramatically reducing the levels of reduced glutathione. This was achieved through reduction in the activity of glutathione-S-transferase. Conclusion: Our findings indicate that the studied preparation of graviola displayed cytotoxic effects on some cells, depending on the dose and length of action.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background: The extracts of Annona muricata, also known as graviola, form part of traditional medicine. Objectives: Verification of their effects and elucidation of their mechanisms is beneficial in terms of the utility of these extracts in treatment or prevention. Methods: We monitored the effectiveness of an extract of dried graviola leaves available for direct consumption on cancer cell lines and isolated rat liver mitochondria. Results: The Jurkat cell line exhibited the highest, sensitivity, with the metabolic activity of cells reaching less than 10% at the highest tested concentration. The effect was dose-dependent. The viability of Jurkat cells was affected after more than the 72 hours. At the same time, there was also a reduction of mitochondrial membrane potential. At 24 to 48 hours incubation, no increase was found in the activity of caspase-3 but increased levels were found for the non-phosphorylated form of Bcl-2. Higher concentrations of graviola affected the mitochondrial redox state by dramatically reducing the levels of reduced glutathione. This was achieved through reduction in the activity of glutathione-S-transferase. Conclusion: Our findings indicate that the studied preparation of graviola displayed cytotoxic effects on some cells, depending on the dose and length of action.
13.
Skalická, Magdaléna; Naď, Pavel; Bujňák, Lukáš; Marcin, Andrej
Impact of Dietary Humic Substances Supplementation on Selected Minerals in Muscles of Broiler Chickens Journal Article
In: Folia Veterinaria, vol. 65, iss. 3, pp. 51-59, 2021.
@article{nokey,
title = {Impact of Dietary Humic Substances Supplementation on Selected Minerals in Muscles of Broiler Chickens},
author = {Magdaléna Skalická and Pavel Naď and Lukáš Bujňák and Andrej Marcin},
doi = {10.2478/fv-2021-0027},
year = {2021},
date = {2021-09-01},
journal = {Folia Veterinaria},
volume = {65},
issue = {3},
pages = {51-59},
abstract = {In this study, we assessed the effect of humic substances on the changes of some mineral (Ca, Mg, Cu and Zn) content in the thigh and breast muscle of broilers. Group 1 (G1) was supplemented with 0.7 % Humac Natur Mycosorb (HNMy), and G2 with 0.3 % HNMy. The control group (GC) received a basal diet without any supplements. In the breast muscle of broilers from the group G1 we found a statistically significantly higher (P < 0.05) Ca content and significantly reduced (P < 0.05) in the thigh muscle Ca content from the group G2 compared to the control group (GC). A statistically significant increase in Mg content (P < 0.05) was analyzed in breast muscles in the group G1 and also significant reduction (P ≤ 0.01) in Cu in the breast and thigh muscles was found in the group G1 and in the breast muscle from the group G2 compared to the GC. Significantly lower content of Zn (P ≤ 0.05) was found in the breast and thigh muscles of broilers in the group G2 and strong positive correlation (r = 0.9093) were observed between Ca in breast muscle from the group G2 and Ca in thigh muscle from the control group. A negative correlation (r = –0.7656) was shown for Mg between thigh muscle from the group G2 and Ca in thigh muscle from the GC. A strong negative correlation was confirmed (r = –0.9221) for Zn content in breast muscles between groups G2 and G1. The high positive correlation for Zn was occurred between breast muscle from the control group and thigh muscle from the group G1 (r = 0.9786).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this study, we assessed the effect of humic substances on the changes of some mineral (Ca, Mg, Cu and Zn) content in the thigh and breast muscle of broilers. Group 1 (G1) was supplemented with 0.7 % Humac Natur Mycosorb (HNMy), and G2 with 0.3 % HNMy. The control group (GC) received a basal diet without any supplements. In the breast muscle of broilers from the group G1 we found a statistically significantly higher (P < 0.05) Ca content and significantly reduced (P < 0.05) in the thigh muscle Ca content from the group G2 compared to the control group (GC). A statistically significant increase in Mg content (P < 0.05) was analyzed in breast muscles in the group G1 and also significant reduction (P ≤ 0.01) in Cu in the breast and thigh muscles was found in the group G1 and in the breast muscle from the group G2 compared to the GC. Significantly lower content of Zn (P ≤ 0.05) was found in the breast and thigh muscles of broilers in the group G2 and strong positive correlation (r = 0.9093) were observed between Ca in breast muscle from the group G2 and Ca in thigh muscle from the control group. A negative correlation (r = –0.7656) was shown for Mg between thigh muscle from the group G2 and Ca in thigh muscle from the GC. A strong negative correlation was confirmed (r = –0.9221) for Zn content in breast muscles between groups G2 and G1. The high positive correlation for Zn was occurred between breast muscle from the control group and thigh muscle from the group G1 (r = 0.9786).
14.
Mudroňová, Dagmar; Karaffová, Viera; Semjon, Boris; Naď, Pavel; Koščová, Jana; Bartkovský, Martin; Makiš, Andrej; Bujňák, Lukáš; Nagy, Jozef; Mojžišová, Jana; Marcinčák, Slavomír
Effects of Dietary Supplementation of Humic Substances on Production Parameters, Immune Status and Gut Microbiota of Laying Hens Journal Article
In: Agriculture, vol. 11, no. 8, pp. 744, 2021.
@article{Mudroov2021,
title = {Effects of Dietary Supplementation of Humic Substances on Production Parameters, Immune Status and Gut Microbiota of Laying Hens},
author = {Dagmar Mudroňová and Viera Karaffová and Boris Semjon and Pavel Naď and Jana Koščová and Martin Bartkovský and Andrej Makiš and Lukáš Bujňák and Jozef Nagy and Jana Mojžišová and Slavomír Marcinčák},
url = {https://doi.org/10.3390/agriculture11080744},
doi = {10.3390/agriculture11080744},
year = {2021},
date = {2021-08-01},
journal = {Agriculture},
volume = {11},
number = {8},
pages = {744},
publisher = {MDPI AG},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
15.
Teter, Anna; Kędzierska-Matysek, Monika; Barłowska, Joanna; Król, Jolanta; Brodziak, Aneta; Florek, Mariusz
The Effect of Humic Mineral Substances from Oxyhumolite on the Coagulation Properties and Mineral Content of the Milk of Holstein-Friesian Cows Journal Article
In: Animals, vol. 11, no. 7, pp. 1970, 2021.
@article{Teter2021,
title = {The Effect of Humic Mineral Substances from Oxyhumolite on the Coagulation Properties and Mineral Content of the Milk of Holstein-Friesian Cows},
author = {Anna Teter and Monika Kędzierska-Matysek and Joanna Barłowska and Jolanta Król and Aneta Brodziak and Mariusz Florek},
url = {https://doi.org/10.3390/ani11071970},
doi = {10.3390/ani11071970},
year = {2021},
date = {2021-06-01},
journal = {Animals},
volume = {11},
number = {7},
pages = {1970},
publisher = {MDPI AG},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
16.
Hudák, Marek; Semjon, Boris; Marcinčáková, Dana; Bujňák, Lukáš; Naď, Pavel; Koréneková, Beáta; Nagy, Jozef; Bartkovský, Martin; Marcinčák, Slavomír
Effect of Broilers Chicken Diet Supplementation with Natural and Acidified Humic Substances on Quality of Produced Breast Meat Journal Article
In: Animals 2021, Vol. 11, Page 1087, vol. 11, iss. 4, pp. 1087, 2021, ISSN: 20762615.
@article{nokey,
title = {Effect of Broilers Chicken Diet Supplementation with Natural and Acidified Humic Substances on Quality of Produced Breast Meat},
author = {Marek Hudák and Boris Semjon and Dana Marcinčáková and Lukáš Bujňák and Pavel Naď and Beáta Koréneková and Jozef Nagy and Martin Bartkovský and Slavomír Marcinčák},
url = {https://www.mdpi.com/2076-2615/11/4/1087/htm https://www.mdpi.com/2076-2615/11/4/1087},
doi = {10.3390/ANI11041087},
issn = {20762615},
year = {2021},
date = {2021-01-01},
journal = {Animals 2021, Vol. 11, Page 1087},
volume = {11},
issue = {4},
pages = {1087},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {This study was conducted to examine the effect of two humic substances (HS) supplemented in broilers’ diet on the breast meat quality of broiler chickens. In this experiment, 120 pieces of one-day-old COBB500 broiler chickens were used. Broilers were divided into three groups, each containing 40 birds with three replications (13, 13, and 14 per one pen). Fattening lasted 38 days. The first experimental diet was supplemented with 0.7% of HS (HS0.7) and the second was enriched with 0.7% of acidified HS (HSA0.7). The control group of broilers (C) was fed a basal diet without the addition of any supplements. HS0.7 samples had the highest total protein content and the lowest content of fat (p < 0.01). The effects of broiler diet and storage had a significant impact on the pH of breast samples, p < 0.001 and p < 0.05, respectively. Supplementation of HS in broiler diet positively affected the lipid oxidation of breast meat samples, as indicated by the lower malondialdehyde content (p < 0.05). HSA0.7 samples had significantly better juiciness after the storage period (p < 0.001). The quality of meat produced with supplementation of HS in broilers’ feed can be considered as valuable in human nutrition due to improved protein and fat content with decreased lipid oxidation.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study was conducted to examine the effect of two humic substances (HS) supplemented in broilers’ diet on the breast meat quality of broiler chickens. In this experiment, 120 pieces of one-day-old COBB500 broiler chickens were used. Broilers were divided into three groups, each containing 40 birds with three replications (13, 13, and 14 per one pen). Fattening lasted 38 days. The first experimental diet was supplemented with 0.7% of HS (HS0.7) and the second was enriched with 0.7% of acidified HS (HSA0.7). The control group of broilers (C) was fed a basal diet without the addition of any supplements. HS0.7 samples had the highest total protein content and the lowest content of fat (p < 0.01). The effects of broiler diet and storage had a significant impact on the pH of breast samples, p < 0.001 and p < 0.05, respectively. Supplementation of HS in broiler diet positively affected the lipid oxidation of breast meat samples, as indicated by the lower malondialdehyde content (p < 0.05). HSA0.7 samples had significantly better juiciness after the storage period (p < 0.001). The quality of meat produced with supplementation of HS in broilers’ feed can be considered as valuable in human nutrition due to improved protein and fat content with decreased lipid oxidation.
17.
Haus, Martin; Žatko, Daniel; Vašková, Janka; Vaško, Ladislav
The effect of humic acid in chronic deoxynivalenol intoxication Journal Article
In: Environmental Science and Pollution Research, vol. 28, iss. 2, pp. 1612-1618, 2021, ISSN: 0944-1344.
@article{Haus2021,
title = {The effect of humic acid in chronic deoxynivalenol intoxication},
author = {Martin Haus and Daniel Žatko and Janka Vašková and Ladislav Vaško},
url = {https://link.springer.com/10.1007/s11356-020-10581-x},
doi = {10.1007/s11356-020-10581-x},
issn = {0944-1344},
year = {2021},
date = {2021-01-01},
journal = {Environmental Science and Pollution Research},
volume = {28},
issue = {2},
pages = {1612-1618},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {The most common mycotoxin found in European foodstuffs, especially unprocessed grains, is deoxynivalenol (DON), which inhibits proteosynthesis and induces oxidative stress. The sorption properties of humic acids (HA) indicate a promising potential in the reduction of mycotoxin intoxication. In an experimental biomodel lasting 35 days, we investigated the effect of a dietary supplement containing HA on the antioxidant status in the liver, heart and kidney mitochondria and in the blood plasma of rats exposed to the stress factor of orally administered DON. DON was administered at doses of 100 and 200% above the maximum tolerable daily intake (1.0 μg/kg body weight/day). We evaluated the activities of the following enzymes: superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase and tripeptide glutathione. Administration of DON has been shown to induce oxidative stress, the intensity of which appears to be directly dependent on the concentration of mycotoxin. Supplementation with 1% HA (10 g/kg of feed) resulted in alleviation of this stress, in which the preventive effect of HA may have been implicated partly by affecting the adsorption of DON through the GIT and limiting its bioavailability. There were also signs that it can act by actively interfering with ROS inhibition to help achieve redox homeostasis. However, there is another aspect that deserves attention, namely, the metabolism of HA. The results presented in our work suggest that the mechanism of possible metabolism occurs predominantly in the liver, including an oxidative increase in polarity and subsequent conjugation reactions mediated by the GST-GSH system.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The most common mycotoxin found in European foodstuffs, especially unprocessed grains, is deoxynivalenol (DON), which inhibits proteosynthesis and induces oxidative stress. The sorption properties of humic acids (HA) indicate a promising potential in the reduction of mycotoxin intoxication. In an experimental biomodel lasting 35 days, we investigated the effect of a dietary supplement containing HA on the antioxidant status in the liver, heart and kidney mitochondria and in the blood plasma of rats exposed to the stress factor of orally administered DON. DON was administered at doses of 100 and 200% above the maximum tolerable daily intake (1.0 μg/kg body weight/day). We evaluated the activities of the following enzymes: superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase and tripeptide glutathione. Administration of DON has been shown to induce oxidative stress, the intensity of which appears to be directly dependent on the concentration of mycotoxin. Supplementation with 1% HA (10 g/kg of feed) resulted in alleviation of this stress, in which the preventive effect of HA may have been implicated partly by affecting the adsorption of DON through the GIT and limiting its bioavailability. There were also signs that it can act by actively interfering with ROS inhibition to help achieve redox homeostasis. However, there is another aspect that deserves attention, namely, the metabolism of HA. The results presented in our work suggest that the mechanism of possible metabolism occurs predominantly in the liver, including an oxidative increase in polarity and subsequent conjugation reactions mediated by the GST-GSH system.
18.
Szewczyk, Agata; Kuszta, Małgorzata
Dodatki funkcjonalne, takie jak sól, kwasy huminowe i mikroelementy – jako modulatory wydajności i dobrego zdrowia wysokowydajnych krów mlecznych Journal Article
In: Wiadomości Zootechniczne, vol. LIX, iss. 4, pp. 16-24, 2021, ISSN: 1731-8068.
@article{nokey,
title = {Dodatki funkcjonalne, takie jak sól, kwasy huminowe i mikroelementy – jako modulatory wydajności i dobrego zdrowia wysokowydajnych krów mlecznych},
author = {Agata Szewczyk and Małgorzata Kuszta},
issn = {1731-8068},
year = {2021},
date = {2021-01-01},
urldate = {2021-01-01},
journal = {Wiadomości Zootechniczne},
volume = {LIX},
issue = {4},
pages = {16-24},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
19.
Holátko, Jiří; Hammerschmiedt, Tereza; Datta, Rahul; Baltazar, Tivadar; Kintl, Antonin; Latal, Oldřich; Pecina, Václav; Sarec, Petr; andLudmila Baláková, Petr Novák; Danish, Subhan; Zafar-ul-Hye, Muhammad; Fahad, Shah; Brtnický, Martin
Humic Acid Mitigates the Negative Effects of High Rates of Biochar Application on Microbial Activity Journal Article
In: Sustainability, vol. 12, iss. 9524, pp. 9524, 2020.
@article{nokey,
title = {Humic Acid Mitigates the Negative Effects of High Rates of Biochar Application on Microbial Activity},
author = {Jiří Holátko and Tereza Hammerschmiedt and Rahul Datta and Tivadar Baltazar and Antonin Kintl and Oldřich Latal and Václav Pecina and Petr Sarec and Petr Novák andLudmila Baláková and Subhan Danish and Muhammad Zafar-ul-Hye and Shah Fahad and Martin Brtnický
},
doi = {10.3390/su12229524},
year = {2020},
date = {2020-11-01},
journal = {Sustainability},
volume = {12},
issue = {9524},
pages = {9524},
abstract = {Biochar and a commercial humic acid-rich product, Humac (modified leonardite), represent soil amendments with the broad and beneficial effects on various soil properties. Their combination has been scarcely tested so far, although the positive impact of their interaction might be desirable. Materials and Methods: The dehydrogenase activity (DHA), microbial biomass carbon (C mic), soil respiration (basal and substrate-induced), enzyme activities, total carbon (C tot), and both shoot and root biomass yield were measured and compared in the short-term pot experiment with the lettuce seedlings. The following treatments were tested: the unamended soil (control), the Humac-amended soil (0.8 g·kg −1), the biochar-amended soil (low biochar 32 g·kg −1 , high biochar 80 g·kg −1), and the soil-amended with biochar + Humac. Results: The effect of both amendments on the soil pH was insignificant. The highest average values of C tot and C mic were detected in high biochar treatment and the highest average values of basal and substrate-induced respiration (glucose, glucosamine, alanine) were detected in the low biochar treatment. The phosphatase activity and fresh and dry lettuce aboveground biomass were the highest in the low biochar + Humac treatment. Conclusions: Even though the combination of both biochar + Humac decreased the microbial activities in the amended soil (C mic , DHA, enzymes, substrate-induced respiration) at the low biochar dose, they mitigated the detrimental effect of the high biochar dose on respiration (all the types) and the enzyme (phosphatase, arylsulphatase) activities. In contrast to the previously published research in this issue, the effects could not be attributed to the change of the soil pH.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Biochar and a commercial humic acid-rich product, Humac (modified leonardite), represent soil amendments with the broad and beneficial effects on various soil properties. Their combination has been scarcely tested so far, although the positive impact of their interaction might be desirable. Materials and Methods: The dehydrogenase activity (DHA), microbial biomass carbon (C mic), soil respiration (basal and substrate-induced), enzyme activities, total carbon (C tot), and both shoot and root biomass yield were measured and compared in the short-term pot experiment with the lettuce seedlings. The following treatments were tested: the unamended soil (control), the Humac-amended soil (0.8 g·kg −1), the biochar-amended soil (low biochar 32 g·kg −1 , high biochar 80 g·kg −1), and the soil-amended with biochar + Humac. Results: The effect of both amendments on the soil pH was insignificant. The highest average values of C tot and C mic were detected in high biochar treatment and the highest average values of basal and substrate-induced respiration (glucose, glucosamine, alanine) were detected in the low biochar treatment. The phosphatase activity and fresh and dry lettuce aboveground biomass were the highest in the low biochar + Humac treatment. Conclusions: Even though the combination of both biochar + Humac decreased the microbial activities in the amended soil (C mic , DHA, enzymes, substrate-induced respiration) at the low biochar dose, they mitigated the detrimental effect of the high biochar dose on respiration (all the types) and the enzyme (phosphatase, arylsulphatase) activities. In contrast to the previously published research in this issue, the effects could not be attributed to the change of the soil pH.
20.
Mudroňová, Dagmar; Karaffová, Viera; Pešulová, T.; Koščová, J.; Maruščáková, I. Cingeľová; Bartkovský, M.; Marcinčáková, Daniela; Ševčíková, Z.; Marcinčák, Slavomír
The effect of humic substances on gut microbiota and immune response of broilers Journal Article
In: Food and Agricultural Immunology, vol. 31, iss. 1, pp. 137-149, 2020, ISSN: 0954-0105.
@article{nokey,
title = {The effect of humic substances on gut microbiota and immune response of broilers},
author = {Dagmar Mudroňová and Viera Karaffová and T. Pešulová and J. Koščová and I. Cingeľová Maruščáková and M. Bartkovský and Daniela Marcinčáková and Z. Ševčíková and Slavomír Marcinčák},
url = {https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1707780},
doi = {10.1080/09540105.2019.1707780},
issn = {0954-0105},
year = {2020},
date = {2020-01-01},
urldate = {2020-01-01},
journal = {Food and Agricultural Immunology},
volume = {31},
issue = {1},
pages = {137-149},
publisher = {Taylor and Francis Ltd.},
abstract = {The work focused on the study of the immunomodulatory and gut-protecting effect of humic substances (HS) in broilers. The diet of experimental chicks was enriched with 0.8% of HS. We noted that HS had a stimulatory effect on the phagocytic activity and the engulfing capacity of phagocytes, however, the level of oxidative burst of phagocytes was not affected. We observed a significant increase of CD4+: CD8+ lymphocyte ratio, an indicator of immune stimulation. HS did not influence the IgA gene expression. In contrast, we observed a significant increase in the expression of MUC-2 (intestinal mucin 2) gene, and a decrease in the expression of IGF-2 (insulin-like growth factor 2) and also AvBD2 (avian beta defensin 2) genes. A decreased Enterobacteriaceae counts in the gut of experimental animals showed a positive effect on intestinal microbiota. We confirmed a gut-protecting and an immunostimulatory effect of HS in broiler chickens.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The work focused on the study of the immunomodulatory and gut-protecting effect of humic substances (HS) in broilers. The diet of experimental chicks was enriched with 0.8% of HS. We noted that HS had a stimulatory effect on the phagocytic activity and the engulfing capacity of phagocytes, however, the level of oxidative burst of phagocytes was not affected. We observed a significant increase of CD4+: CD8+ lymphocyte ratio, an indicator of immune stimulation. HS did not influence the IgA gene expression. In contrast, we observed a significant increase in the expression of MUC-2 (intestinal mucin 2) gene, and a decrease in the expression of IGF-2 (insulin-like growth factor 2) and also AvBD2 (avian beta defensin 2) genes. A decreased Enterobacteriaceae counts in the gut of experimental animals showed a positive effect on intestinal microbiota. We confirmed a gut-protecting and an immunostimulatory effect of HS in broiler chickens.