Publicaciones cientificas

@article{nokey,

title = {The effects of dietary probiotics and humic acid on meat quality of Japanese quail including sex-related differences and economical background},

author = {Anna Kalafova and Cyril Hrnčár and Katarina Zbynovska and Ondrej Bučko and Emilia Hanusova and Zuzana Kapustova and Monika Schneidgenova and Peter Bielik and Marcela Capcarova},

doi = {10.2478/s11756-018-0085-7},

year  = {2018},

date = {2018-06-14},

journal = {Biologia},

volume = {73},

issue = {8},

pages = {765 - 771},

abstract = {The effects of humic substances (HS) on the quality traits (colour, water-holding capacity, and pH), serum minerals (calcium, phosphorus, sodium, potassium, chlorides) and on the serum metabolites (glucose, total protein, triglycerides and cholesterols) of breast and thigh meats of Japanese quails were studied. A total of 60 birds (30 males and 30 females) were involved in the experiment. The animals were fed by the standard basal diet and divided to the 6 groups as follows: probiotic females (PF, n = 10) and probiotic males (PM, n = 10) received probiotic preparation in the single dose of 1 g/1 kg of feed mixture, humic acids females (HF, n = 10) and humic acid males (HM, n = 10) received humic acids in the single dose 3 g/1 kg of feed mixture. The group fed basal diet without any additive served as the control group (CF; n = 10, CM, n = 10). After 210 days the animals were slaughtered, blood samples and samples of muscles (breast and thigh) were collected. Probiotic and humic acids treatment resulted in the significant increase of serum calcium levels in the female groups against the male groups. The content of serum phosphorus significantly increased in the PF group in comparison to the PM group. In this study both treatments (probiotics and humic acids) significantly decreased amount of HDL cholesterol in the female groups in comparison to the female control. Significant sex-based differences were observed for HDL cholesterol (CF, PF, HF vs. CM, PM, HM) and LDL cholesterol (HM vs. HF). 24 h after slaughter meat pH showed significant differences in different muscles. Other investigated parameters of Japanese quails were not influenced by the treatment. We can conclude, that the effect of probiotics and humic acids treatment seems to be dose dependent and estimation of effective dose of additives used in poultry feeding play an important role. Further studies are needed.},

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pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of humic substances on the reproduction parameters of farmed brown hare: Short communication},

author = {Tomáš Sládeček and Rastislav Jurcik and J. Slamečka and Ľ. Ondruška},

issn = {13379984},

year  = {2018},

date = {2018-05-09},

journal = {Slovak Journal of Animal Science},

volume = {51},

number = {2},

pages = {86-90},

abstract = {The aim of the study was to evaluate the effect of humic acids on the reproduction parameters of farmed brown hares and the possibility of their utilisation as prevention of gastrointestinal tract diseases when feeding green fodder. Animals were divided into three groups, 4 breeding pairs per group. Brown hares in the control group were fed granulated feed mixture without additives. The hares in experimental groups were fed granulated feed mixture with HUMAC Natur additive, purchased from Humacon, at 1 % concentration. The experimental group 2 was fed also green fodder during the entire experiment (April - September) in addition to the granulated feed mixture. In the experimental group 1, the ratio of live born leverets was significantly higher by 19.54 % (P ≤ 0.05) and the ratio of weaned leverets was higher by 9.74 % compared to the control group, but no significant differences were found. The significantly highest mortality (12.50 %; P ≤ 0.001) before weaning (28 days of age) was determined in the experimental group 1. In the experimental group 2 was the highest ratio of weaned leverets (89.19; no significant differences was found) and no increase in mortality, as a result of feeding green fodder, was determined.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of humic acids on poultry under stress conditions},

author = {Janka Vašková and Peter Patlevič and Daniel Žatko and Slavomír Marcinčák and Ladislav Vaško and Klára Krempaská and Jozef Nagy },

url = {https://slovetres.si/index.php/SVR/article/view/469},

doi = {10.26873/SVR-469-2018},

issn = {1580-4003},

year  = {2018},

date = {2018-01-01},

urldate = {2018-01-01},

journal = {SLOVENIAN VETERINARY RESEARCH},

volume = {55},

issue = {4},

publisher = {University of Ljubljana, Veterinary Faculty},

abstract = {The transportation of chickens from the poultry farm to the slaughterhouse causes stress conditions that influence the oxidative status of the whole organism and subsequently change the organoleptic properties of the meat delivered to the consumer. The aim of this work was to investigate how administering 0.6% humic acids to broiler chickens for a period of 42 days affects the level of selected enzymes directly involved in oxidative stress elimination. For the most objective estimation of the oxidative state, parameters were determined in liver and kidney mitochondria, and in the blood plasma. With regards to the chelating properties of humic acids, our interest was in monitoring the effects on the distribution of the transition metals Fe, Zn, Cu, Mn, which serve as cofactors of antioxidant enzymes. We have found that, under normal conditions, 42 days of humic acid administration do not cause significant metal redistribution. It has a significant effect on Se excretion, according to the pronounced deposition of Se in kidney tissue, without significantly increased activity of the corresponding enzyme. This led to compensation by changes in other antioxidant enzyme activities. This is a noteworthy finding, especially after administration of longer than 42 days. In conditions caused by sudden stress, according to the detected element levels, it is possible to expect a better response in the case of humic acid administration. The effect of humic acid supplementation appeared to be organ-specific and may ultimately be beneficial for the chickens' health, stress elimination and, finally, the quality of the meat. Key words: antioxidant enzymes; humic acids; chicken; metal cofactors; oxidative stress UČINKI HUMINSKE KISLINE NA PERUTNINO V STRESNIH POGOJIH Povzetek: Prevoz piščancev s perutninske farme v klavnico povzroča stresne razmere, ki vplivajo na oksidativni status celotnega organizma in kasneje spremenijo organoleptične lastnosti mesa, dostavljenega potrošniku. Cilj raziskave je bil proučiti, ali dodajanje 0,6 % huminske kisline v hrano pitovnim piščancem za 42 dni vpliva na aktivnost nekaterih encimov, ki so neposredno vključeni v urejanje in zmanjševanje oksidativnega stresa. Za objektivno ocenjevanje oksidativnega stanja so se določevali parametri v jetrnih in ledvičnih mitohondrijih ter v krvni plazmi. Kelacijske lastnosti huminske kisline so bile proučevane s spremljanjem učinkov na porazdelitev prehodnih kovin Fe, Zn, Cu, Mn, ki služijo kot kofaktorji antioksidantnih encimov. Ugotovili so, da v normalnih pogojih 42-dnevno dodajanje huminske kisline ne povzroči bistvene prerazporeditve kovin. Dodajanje pa pomembno vpliva na izločanje Se glede na izrazito usedlino Se v tkivu ledvic, brez bistveno povečane aktivnosti ustreznega encima. To je privedlo do sprememb, ki so nadomestile aktivnosti drugih antioksidantnih encimov. To je pomembna ugotovitev, še posebej pri dodajanjih več kot 42 dni. V pogojih, ki jih povzroča nenadni stres, je glede na ugotovljene ravni elementov mogoče pričakovati boljši odziv pri uporabi huminske kisline. Učinek dopolnjevanja s huminsko kislino se je izkazal za organsko-specifičnega in je lahko koristen za zdravje piščancev, odpravo stresa in končno kakovost mesa. Ključne besede: antioksidativni encimi; humične kisline; piščanec; kovinski kofaktorji; oksidativni stres},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The impact of the humic acid and phytobiotics on performance and carcass parameters of broiler chickens},

author = {Henrieta Arpášová and Veronika Pistová and Cyril Hrnčár and Martin Fik and Peter Haščík and Miroslava Kačániová and Branislav Gálika and Ondrej Bučko},

doi = {10.15414/afz.2018.21.04.173-178},

issn = {1335-258X},

year  = {2018},

date = {2018-01-01},

urldate = {2018-01-01},

journal = {Acta fytotechnica et zootechnica},

volume = {21},

issue = {4},

pages = {173-178},

abstract = {The aim of this experiment was to determine the influence of humic substances or humic substances and thyme on carcass parameters of broiler chickens. A total of 60 Ross 308 broiler chickens were divided into 3 groups (n=20). The control group of chickens was fed with complete feed mixtures without any additives. Chickens in the first experimental group were fed a diet containing 1.5% of humic substances. Chickens in the second experimental group were fed a diet containing 1.5% of humic substances and 0.5% of thyme. In 42 day of fattening, 5 male and 5 females with body weight similar to the mean were chosen from each group of chickens. The carcass weight (g), the breast and thigh percentages (%), edible weight (g) and carcass yield (%) were recorded. We found statistical significant increase (P<0.05) of heart weight in the first experimental group in comparison with other groups. The favourable tendency of the effect of the supplements used, although without a statistically significant differences (P>0.05), was observed in both experimental groups in liver and gizzard weights. We recorded statistically non-significant differences (P>0.05) in carcass weight, carcass yield, breast a thigh percentage in the both experimental groups compared with control group.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The Effect of the Humic Substances and Thyme on Carcass Parameters of Broiler Chickens},

author = {Henrieta Arpášová and Veronika Pistová and Cyril Hrnčár and Martin Fik},

year  = {2018},

date = {2018-01-01},

journal = {Scientific Papers: Animal Science & Biotechnologies},

volume = {51},

issue = {2},

abstract = {The aim of this experiment was to determine the influence of humic substances or humic substances and thyme on carcass parameters of broiler chickens. A total of 60 Ross 308 broiler chickens were divided into 3 groups (n=20). The control group of chickens was fed with complete feed mixtures without any additives. Chickens in the first experimental group were fed a diet containing 1.5% of humic substances. Chickens in the second experimental group were fed a diet containing 1.5% of humic substances and 0.5% of thyme. In 42 day of fattening, 5 male and 5 females with body weight similar to the mean were chosen from each group of chickens. The carcass weight (g), the breast and thigh percentages (%), edible weight (g) and carcass yield (%) were recorded. We found statistical significant increase (P<0.05) of heart weight in the first experimental group in comparison with other groups. The favourable tendency of the effect of the supplements used, although without a statistically significant differences (P>0.05), was observed in both experimental groups in liver and gizzard weights. We recorded statistically nonsignificant differences (P>0.05) in carcass weight, carcass yield, breast a thigh percentage in the both experimental groups compared with control group.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effectiveness of non-antibiotic stimulators in Japanese quail diet: Gender comparison and economical annex},

author = {Marcela Capcarova and Anna Kalafova and Zuzana Lajdova and Marianna Schwarzova and Katarina Zbynovska and Cyril Hrnčár and Emilia Hanusova and Zuzana Brunaiova and Peter Bielik},

doi = {10.1515/biolog-2017-0004},

year  = {2017},

date = {2017-12-30},

journal = {Biologia},

volume = {72},

pages = {96–104},

abstract = {Economical as well as medicinal and nutritional benefits of Japanese quails are important. The aim of the present study was to investigate the effect of dietary inclusion of probiotics and humic acids on some antioxidant, carcass and egg production parameters of Japanese quails. As Japanese quails are an interesting animal model attracting attention in the last years, a partial aim of this study is also a brief economical annex to the topic. A total of 60 birds (30 males and 30 females, age of 35 days) were involved in the experiment. Animals were fed by standard basal diet and divided to 6 experimental groups as follows: two groups (10 females and 10 males) received addition of probiotics preparation in a single dose of 1 g kg−1 of feed mixture, two groups (10 females and 10 males) received a preparation of humic acids in a single dose of 3 g kg−1 of feed mixture. The groups (10 females and 10 males) fed on basal diet without any additive served as the control group. After 210 days the animals were euthanized and blood samples, carcass organs and abdominal fat were collected. Antioxidant/oxidant parameters (activity of superoxide dismutase SOD, glutathione peroxidase GPx, total antioxidant status TAS, content of bilirubin and reactive oxygen species ROS) from blood samples were measured. The production of eggs was recorded during the experiment. Probiotic and humic acids treatments resulted in significant increase of TAS in females in contrast to males. Content of bilirubin was significantly lower in females fed with addition of humic acids in comparison to the male group. In both treatments (probiotics and humic acids) significantly lower amount of abdominal fat in females in comparison to the controls were recorded. The other investigated parameters of Japanese quails were not influenced by the treatments. The effect of probiotics and humic acids is probably dose dependent and the assessment of an accurate dose of substantives used in poultry feeding plays a crucial role.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Wpływ kwasów huminowych w dawkach dla owiecna aktywność enzymatyczną żwacza},

author = {Małgorzata P. Majewska and Renata Miltko and Jacek Skomiał and Barbara Kowalik},

doi = {10.21521/mw.5822},

year  = {2017},

date = {2017-12-01},

urldate = {2017-12-01},

journal = {Medycyna Weterynaryjna},

volume = {73},

issue = {12},

pages = {770-773},

abstract = {The objective of the present study was to investigate how different levels of natural humic acid in the diet affect the hydrolases activity in the rumen of sheep. The experiment was carried out on three rumen-fistulated adult sheep. The control animals were fed a diet (K) containing 59.7% meadow hay, 28.4% crushed barley, 9.8% soybean oil meal and 2.1% vitamin mineral mixture. The experimental diets were composed of the control ration supplemented with humic acid (HUMAC natur AFM® containing 65% humic acid in dry matter) and supplied at doses of 10 g · d–1 (diet H10) or 20 g · d–1 (diet H20). Samples of rumen digesta were collected just before the morning feeding, as well as 2, 4, and 8 h afterwards. In the present study, the cellulolytic activity in the rumen digesta was higher (P < 0.01) in sheep fed the K diet than it was in animals fed the H20 ration 2 h after feeding. However, the effect of humic acid on the cellulose degradation rate depended on the content of humic acid in the diet. The cellulolytic activity was increased 2 h after feeding in sheep fed the H10 diet as compared with animals fed the H20 ration (P < 0.05). Sampling time had significant influence on the hydrolases activity in the rumen of sheep. In sheep fed the H10 ration, the cellulolytic activity was higher 8 h after feeding than it was before feeding and 4 h after feeding (P < 0.05). In the H10 and H20 groups, the xylnalolytic activity was higher before feeding, but lower than it was in the control group K. The degradation rates of xylan were decreased 2 and 4 h after feeding in sheep fed the H10 and H20 rations and then increased 8 h after feeding (P < 0.05). In sheep fed the H20 diet, the amylolitic activity 8 h after feeding was higher than it was 2 h after feeding (P < 0.05). The effect of humic acid on the hydrolases activity in the rumen of sheep is not uniform and depends on the quantity of humic acid in the diet and on sampling time. Humic acid present in commercial HUMAC natur AFM® product enhances the initial rate of cellulose degradation in the rumen at a dose of 10 g · d-1. Thus, it may suggest that humic acid stimulates the growth of fibrolytic microorganisms in the rumen. On the other hand, the present results suggest that humic acid may reduce the rate of carbohydrate degradation in the rumen, since a higher hydrolases activity was observed before morning feeding and 8 h afterwards.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The Effect of Natural Substance HUMAC Enviro on Reducing the Concentration of Copper and Mercury in Contaminated Soils},

author = {Danica Fazekašová and Juraj Fazekaš and Petra Semancová and Eva Benková and Miroslav Karahuta},

doi = {10.15344/2455-2372/2016/125},

issn = {2455-2372},

year  = {2016},

date = {2016-10-20},

journal = {International Journal of Metallurgical & Materials Engineering},

volume = {2},

issue = {125},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The use of natural substances as a contribution to solving the revitalization of contaminated areas},

author = {Juraj Fazekaš and Danica Fazekašová and Petra Semancová and Jana Chovancová},

url = {https://www.scientific-publications.net/en/article/1001331/},

year  = {2016},

date = {2016-09-30},

journal = {Ecology & Safety},

volume = {10},

pages = {487-492},

abstract = {The paper presents the results of experimental studies focused on possible solutions of the revitalization of soils contaminated by copper mining on the territory of Central Spiš (Slovakia). We have tested natural stimulator based on humic and fulvic acids - HUMAC Enviro in laboratory conditions. The test results showed that the copper content in the aqueous solution coming from the contaminated soils exceeded the permitted limits set out by the law 13 times. After the application of the natural stimulator the copper content has been significantly decreased in five days interval by 40% and the average value of Cu exceeded the limiting values set out by the law 8 times. In response to this finding, as well as numerous global researches aimed at exploring the impact of humic acids in environmental compartments, application of natural substance HUMAC Enviro seems to be important in addressing current environmental problems related to environmental contamination.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Wpływ Humacu Agro na plon, zawartość cukru  w burakach cukrowych i właściwości gleby  w warunkach zrównoważonego systemu rozwoju rolnictwa},

author = {Štefan Tóth and Wojciech Rysak and Barbara Symanowicz and Božena Šoltysová and Jaroslav Karahuta},

doi = {10.24326/as.2016.1.7},

year  = {2016},

date = {2016-04-01},

journal = {Agronomy Science},

volume = {71},

issue = {1},

pages = {77-86},

abstract = {W pracy przedstawiono wyniki badań bezpośredniego wpływu Humacu Agro na plon, zawartość cukru w burakach cukrowych i wybrane właściwości gleby w warunkach klimatyczno-glebowych południowo-wschodniej Polski. Humac Agro zastosowano wczesną wiosną. W badaniach uwzględniono trzy warianty: obiekt kontrolny (V1), 250 kg×ha-1 Humac Agro (V2) i 500 kg×ha-1 Humac Agro (V3). Nawożenie azotem w dawce 60,3 kg×ha-1 stosowano na obiekcie V3, a 94,8 kg×ha-1 na obiekcie V1 i V2. Dawki nawozów PKS stosowano takie same dla wszystkich wariantów nawozowych. Największy plon buraków cukrowych (95,97 Mg×ha-1) uzyskano z wariantu V3. Humac Agro w dawce 500 kg×ha-1 wpłynął na zwiększenie plonu cukru o 29,6% w odniesieniu do obiektu kontrolnego. Zwiększyła się również zawartość próchnicy w glebie, pH gleby i zawartość przyswajalnych form P, K, Mg, B, Mn, Cu, Zn i Fe.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Humic Acids as Therapeutic Compounds in Lead Intoxication},

author = {Klára Krempaská and Ladislav VaŠko and Janka VaŠková},

url = {http://www.eurekaselect.com/openurl/content.php?genre=article&issn=1574-8847&volume=11&issue=3&spage=159},

doi = {10.2174/1574884711666160813233225},

issn = {15748847},

year  = {2016},

date = {2016-01-01},

journal = {Current Clinical Pharmacology},

volume = {11},

issue = {3},

pages = {159-167},

abstract = {Background: Understanding the physicochemical basis and the different models of nanosystems is nowadays fundamental in a great number of scientific areas and industrial processes. Results: Here, we focus on nanosystems created by self-assembly, molecular or inorganic. The organization of single units at these scales is a challenging matter in light of the inherently small dimensions involved, the sensitivity of the system to small perturbations, and the problem of scaling up such a process for widespread use and implementation. Conclusion: This review examines the different self-assembly routes used to create nanostructures in both the equilibrium and non-equilibrium/dynamic systems and discusses their limits and applications. The connection to biomedicine and pharmaceutical design has been emphasized.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of Probiotics and Humic Acid on Egg Production and Quality Parameters of Laying Hens Eggs},

author = {Henrieta Arpášová and Miroslava Kačániová and Veronika Pistová and Branislav Gálika and Martin Fik and Lukáš Hleba},

issn = {2344 - 4576},

year  = {2016},

date = {2016-01-01},

journal = {Animal Science & Biotechnologie},

volume = {49},

issue = {2},

pages = {1-9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The content of dietary fiber, amino acids, dihydroxyphenols and some macro- and micronutrients in grain of conventionally and organically grown common wheat, spelt wheat and Proso Millet},

author = {Cezary Andrzej Kwiatkowski and Małgorzata Haliniarz and Marta Tomczyńska-Mleko and Stanisław Mleko and Małgorzata Kawecka-Radomska},

doi = {10.23986/afsci.50953},

year  = {2015},

date = {2015-10-31},

journal = {Agricultural and Food Science},

volume = {24},

number = {3},

pages = {195-205},

abstract = {The effects of conventional and organic farming system on some quality parameters of grains were studied in winter and spring common wheat, spelt wheat and proso millet. Under organic farming conditions, spelt wheat was characterized by the most favorable grain chemical composition (essential amino acids index [EAAI] 85.3, o-dihydroxyphenol 2.00 g kg-1, nitrogen [N] 23.5 g kg-1, magnesium [Mg] 705, zinc [Zn] 32.9 mg kg-1), followed by millet (total dietary fiber [TDF] 185.3 g kg-1, Mg 904, copper [Cu] 6.27, iron [Fe] 57.0 mg kg-1). The above-mentioned cereals also showed a satisfactory yield level under the organic system (spelt wheat 2.69, proso millet 1.42 t ha-1). Both in winter and spring common wheat organic farming led a significant reduction in productivity, but the content of chemical components in grain (dihydroxyphenols—spring wheat 1.68 g kg-1, winter wheat 1.74 g kg-1; selenium [Se]—spring wheat 53.4 mg kg-1, winter wheat 40.5 mg kg-1; some amino acids—spring wheat valine [Val] 5.11, methionine [Met] 2.09, tryptophan [Trp] 0.40 g kg-1, winter wheat glutamate [Glu] 41.9, proline [Pro] 15.3, glysine [Gly] 5.24, arginine [Arg] 5.04, [Trp] 0.97 g kg-1) was more favorable compared to the conventional system. The present study showed that the organic farming system does not result in reduced productivity neither in spelt wheat or proso millet, but contributes to an improvement in their grain quality parameters. On the other hand, common wheat performed better under the conventional system.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of soil coditioner based on humic acids HUMAC Agro on soil and yield and sugar content of sugar beet in context of selected indicators of agriculture system sustainability},

author = {Štefan Tóth and Wojciech Rysak and Božena Šoltysová and Jaroslav Karahuta},

issn = {1805-9708},

year  = {2015},

date = {2015-09-01},

journal = {Listy cukrovarnické a řepařské},

number = {131},

abstract = {This work evaluates the results of direct effect of HUMAC Agro on

yield and sugar content of sugar beet as well as on selected soil

properties in a field trial established in soil-climatic conditions of

south Poland in 2013 (vegetation season: average air temperature

16.0 o

C, total precipitations 261 mm). Soil conditioner HUMAC

Agro was applied to middle heavy pseudopodzolic soil in early

spring of 2013 in two dosages: 500 kg ha–1 in case of Variant 1 (V1)

and 250 kg ha–1 in case of Variant 2 (V2); it was not applied in

case of Variant 3 (V3). Nitrogen fertilization according to Variants:

60.3 kg ha–1 N for V1, 94.8 kg ha–1 N for both V2 and V3. Doses of

phosphorus, potassium and sulphur were the same for all Variants.

V1 achieved the highest sugar beet yield 95.97 t ha–1 with sugar

content 18.12 % which meant the highest sugar yield 17.39 t ha–1

(+29.6 % more than control treatment). V2 achieved sugar beet

yield 86.39 t ha–1 with the lowest sugar content 17.65 % and sugar

yield 15.25 t ha–1 (+13.6 % more than control treatment). The

control treatment achieved the lowest sugar beet yield 72.81 t ha–1

with the highest sugar content 18.43 % and the lowest sugar yield

13.42 t ha–1. Soil conditioner HUMAC Agro also influenced soil

properties. After the application of 500 kg ha–1 HUMAC Agro the

humus content in soil and its pH increased (due to buffering effect)

as well as contents of the available forms of all observed nutrients

(P, K, Mg, B, Mn, Cu, Zn and Fe) in spite of higher sugar beet yield

and thus higher nutrient uptake compared to the control treatment.

The mentioned soil properties increase applied also when compared to the state in the beginning. The higher humus content may

be caused by the increased yields (besides the conditioner humic

acids input), due to higher formation of root mass, which remains

in the soil after harvesting the crop. Conversely, higher content

of available forms of solved nutrients in the soil usually correlates

with their lower consumption and lower harvest. Higher nutrient

uptake by higher yields and higher soil nutrients content at the

same time is a desirable effect of soil conditioners that prevent

leaching of mobile nutrients and increase the accessibility of

nutrients from their unavailable forms in the soil. The mentioned

desirable effect is not obvious in any soil and climatic conditions,

so it is therefore necessary to quantify the effect of soil conditioners on the soil properties by laboratory analyses especially to

prevent a decline in nutrients due to increased yields. The values

of monitored soil parameters for the control variant compared

to their initial state showed decrease with the exception of Mn

and Fe contents.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Impact of humic acids on trace element content under different conditions},

author = {Janka Vašková and Peter Patlevič and Daniel Žatko and Ladislav Vaško and Slavomír Marcinčák},

issn = {0015-5748},

year  = {2015},

date = {2015-01-01},

journal = {Folia Veterinaria},

volume = {59},

issue = {3},

pages = {159-164},

abstract = {The observations in mitochondria share a lot of similarities in trace element distribution with those observed previously in chicken bodies after humic acids supplementation. The redistribution in the levels of some elements, especially the overall increase in iron and decrease in selenium, could have important effects on the use of elements and the overall redox state of the organism. Based on our findings, it is necessary to consider the length of safe use, in addition to the level of dosage. After 42-days of supplementation with humic acid, according to the levels of trace elements found, an improved response of the organism to stress conditions can be expected.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of supplementation of the diet with humic acids on growth performance and carcass yield of broilers},

author = {Dana Marcinčáková and Ján Mačanga and Jozef Nagy and Slavomír Marcinčák and Peter Popelka and Janka Vašková and Iveta Jaďuttová and Martin Mellen},

issn = {0015-5748},

year  = {2015},

date = {2015-01-01},

urldate = {2015-01-01},

journal = {Folia Veterinaria},

volume = {59},

issue = {3},

pages = {165-169},

abstract = {The aim of this study was to confirm the effects of

the product HUMAC Natur (humic acid source) supplemented to broiler feed during 39 days of fattening on the;

final body weight, carcass yield and percentage of the carcasses. Eighty broilers COBB 500 included in the experiment were fed commercial diet. Humic acids were added

to the feed of experimental broilers (n=40) as a  0.6%

concentrate, from the first day of fattening. On day  39

of fattening, the mean body weight of the experimental

broilers was lower (2498.0±244.6g) in comparison to the

control (2535.0±268.2g) (P>0.05). The addition of the

humic acid product to feed as a 0.6% concentrate affected

positively the carcass yield (P<0.05), but had no significant positive effect on the live body weight (P>0.05). The

mean weight of the breast and thigh muscles in the experimental and control groups was comparable.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The Effect of Humic Acid on the Content of Trace Element in Mitochondria},

author = {Daniel Žatko and Janka Vašková and Ladislav Vaško and Peter Patlevič},

url = {http://thescipub.com/abstract/10.3844/ajavsp.2014.315.319},

doi = {10.3844/ajavsp.2014.315.319},

issn = {1557-4555},

year  = {2014},

date = {2014-01-01},

journal = {American Journal of Animal and Veterinary Sciences},

volume = {9},

issue = {4},

pages = {315-319},

abstract = {Based on our previous in vitro studies which pointed to the antioxidant properties of humic acids especially concerning radical scavenging activities and ability to maintain the redox system in the cell, an addition of 0.6% humic acid into the diet of farm chicken for 42 days was evaluated. The administration of humic acids have a positive effect on the antioxidant status of plasma and mitochondria of important synthetic, metabolic and organs directly involved in the elimination of oxidative stress conditions-the liver, kidney. With regard to the described chelating properties of humic acid, the current levels of iron, copper, manganese, zinc and selenium considering their functions as cofactors in mitochondria was investigated, too. Our finding incline to an expectation that the administration of humic acid does not affect the binding abilities to metals but rather competition, leading to a decline in selenium use and compensatory responses which should be considered especially when administered over 42 days.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effects of humic acids in vitro},

author = {Janka Vašková and Beáta Veliká and Martina Pilátová and Ivan Kron and Ladislav Vaško},

url = {http://link.springer.com/10.1007/s11626-011-9405-8},

doi = {10.1007/s11626-011-9405-8},

issn = {1071-2690},

year  = {2011},

date = {2011-01-01},

journal = {In Vitro Cellular & Developmental Biology - Animal},

volume = {47},

issue = {5-6},

pages = {376-382},

abstract = {Humic acids are known for their overall positive health and productivity effects in animal feeding trials and, controversially, as an aetiological factor of cancer. We tried to assess the in vitro effect of humic acids from a selected source in Slovakia when used at recommended prophylactic dosage. We investigated antioxidant properties, enzymatic and non-enzymatic antioxidant defence system in liver mitochondria and cultured cancer cell lines in vitro. We observed a significant decrease in superoxide dismutase activity after humic acids treatment irrespective of dissolving in dimethyl sulphoxide or direct addition to mitochondria suspension in a respiration medium. Activities of other antioxidant enzymes measured, such as glutathione peroxidase and glutathione reductase, showed no significant differences from the control as well as the reduced glutathione content. Percentage of inhibition by humic acids of superoxide radical indicated lower efficacy compared with that of hydroxyl radical. Survival of six different cancer cells lines indicated that only the acute T lymphoblastic leukaemia cell line was sensitive to the tested humic acids. Despite relatively low solubility in aqueous solutions, humic acids from the selected source participated in redox regulation. By recapturing the radicals, humic acids reloaded the antioxidant defensive mechanism. Results from in vitro study conducted with humic acids from the natural source showed potential of these substances as promising immunity enhancing agents. © 2011 The Society for In Vitro Biology.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}