Tudományos publikációk

@article{article,

title = {Assessment of the effects of Annona muricata leaf aqueous extract in vitro},

author = {Liliána Buzogáňová and Martin Kello and Martina Bago and Daniela Ogurčáková and Janka Vašková},

doi = {10.9755/ejfa.2021.v33.i11.2785},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Emirates Journal of Food and Agriculture},

volume = {33},

issue = {11},

pages = {909-916},

abstract = {Background: The extracts of Annona muricata, also known as graviola, form part of traditional medicine. Objectives: Verification of their effects and elucidation of their mechanisms is beneficial in terms of the utility of these extracts in treatment or prevention. Methods: We monitored the effectiveness of an extract of dried graviola leaves available for direct consumption on cancer cell lines and isolated rat liver mitochondria. Results: The Jurkat cell line exhibited the highest, sensitivity, with the metabolic activity of cells reaching less than 10% at the highest tested concentration. The effect was dose-dependent. The viability of Jurkat cells was affected after more than the 72 hours. At the same time, there was also a reduction of mitochondrial membrane potential. At 24 to 48 hours incubation, no increase was found in the activity of caspase-3 but increased levels were found for the non-phosphorylated form of Bcl-2. Higher concentrations of graviola affected the mitochondrial redox state by dramatically reducing the levels of reduced glutathione. This was achieved through reduction in the activity of glutathione-S-transferase. Conclusion: Our findings indicate that the studied preparation of graviola displayed cytotoxic effects on some cells, depending on the dose and length of action.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Assessing the potential of biochar aged by humic substances to enhance plant growth and soil biological activity},

author = {Tereza Hammerschmiedt and Jiří Holátko and Václav Pecina and Dalibor Huska and Oldřich Latal and Antonin Kintl and Maja Radziemska and Sadiq Muhammad and Zygmunt M. Gusiatin and Martina Kolackova and Muhammad Nasir and Tivadar Baltazar and Niaz Ahmed and Martin Brtnický},

doi = {10.1186/s40538-021-00242-7},

year  = {2021},

date = {2021-09-08},

journal = {Chemical and Biological Technologies in Agriculture},

volume = {8},

number = {46},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Impact of Dietary Humic Substances Supplementation on Selected Minerals in Muscles of Broiler Chickens},

author = {Magdaléna Skalická and Pavel Naď and Lukáš Bujňák and Andrej Marcin},

doi = {10.2478/fv-2021-0027},

year  = {2021},

date = {2021-09-01},

journal = {Folia Veterinaria},

volume = {65},

issue = {3},

pages = {51-59},

abstract = {In this study, we assessed the effect of humic substances on the changes of some mineral (Ca, Mg, Cu and Zn) content in the thigh and breast muscle of broilers. Group 1 (G1) was supplemented with 0.7 % Humac Natur Mycosorb (HNMy), and G2 with 0.3 % HNMy. The control group (GC) received a basal diet without any supplements. In the breast muscle of broilers from the group G1 we found a statistically significantly higher (P < 0.05) Ca content and significantly reduced (P < 0.05) in the thigh muscle Ca content from the group G2 compared to the control group (GC). A statistically significant increase in Mg content (P < 0.05) was analyzed in breast muscles in the group G1 and also significant reduction (P ≤ 0.01) in Cu in the breast and thigh muscles was found in the group G1 and in the breast muscle from the group G2 compared to the GC. Significantly lower content of Zn (P ≤ 0.05) was found in the breast and thigh muscles of broilers in the group G2 and strong positive correlation (r = 0.9093) were observed between Ca in breast muscle from the group G2 and Ca in thigh muscle from the control group. A negative correlation (r = –0.7656) was shown for Mg between thigh muscle from the group G2 and Ca in thigh muscle from the GC. A strong negative correlation was confirmed (r = –0.9221) for Zn content in breast muscles between groups G2 and G1. The high positive correlation for Zn was occurred between breast muscle from the control group and thigh muscle from the group G1 (r = 0.9786).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Mudroov2021,

title = {Effects of Dietary Supplementation of Humic Substances on Production Parameters, Immune Status and Gut Microbiota of Laying Hens},

author = {Dagmar Mudroňová and Viera Karaffová and Boris Semjon and Pavel Naď and Jana Koščová and Martin Bartkovský and Andrej Makiš and Lukáš Bujňák and Jozef Nagy and Jana Mojžišová and Slavomír Marcinčák},

url = {https://doi.org/10.3390/agriculture11080744},

doi = {10.3390/agriculture11080744},

year  = {2021},

date = {2021-08-01},

journal = {Agriculture},

volume = {11},

number = {8},

pages = {744},

publisher = {MDPI AG},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Teter2021,

title = {The Effect of Humic Mineral Substances from Oxyhumolite on the Coagulation Properties and Mineral Content of the Milk of Holstein-Friesian Cows},

author = {Anna Teter and Monika Kędzierska-Matysek and Joanna Barłowska and Jolanta Król and Aneta Brodziak and Mariusz Florek},

url = {https://doi.org/10.3390/ani11071970},

doi = {10.3390/ani11071970},

year  = {2021},

date = {2021-06-01},

journal = {Animals},

volume = {11},

number = {7},

pages = {1970},

publisher = {MDPI AG},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Influence of humic acids, irrigation and fertilization on potato yielding in organic production},

author = {M. Kołodziejczyk},

doi = {10.15159/ar.21.099},

year  = {2021},

date = {2021-05-21},

journal = {Agronomy Research},

volume = {19},

issue = {2},

pages = {520-530},

abstract = {The study aimed at determining the impact of organic fertilization, humic acids and irrigation on potato yielding in organic production system. Fertilization variants included: Humac Agro; manure; vermicompost; Fertil Bioilsa C-N 40-12.5; manure + Humac Agro; vermicompost + Humac Agro; Fertil Bioilsa C-N 40-12.5 + Humac Agro. Irrigation was carried out using drip lines. The highest tuber yield was determined on treatments fertilized with vermicompost. The combined application of organic fertilizers and humic acids resulted in increased total yield between 6 and 9%, whereas commercial yield from 5 to 10%. Application of fertilization resulted in increased total yield of tubers in individual fertilizer variants from 1.9 to 10.8 t ha-1, and commercial yield from 1.6 to 12.3 t ha-1. Water-use efficiency remained in the range from 35.2 to 113.1 kg mm-1, whereas irrigation water-use efficiency from 9.9 to 166.3 kg mm-1. Humic acids used in the study enhanced fertilizer and water-use efficiency. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of Broilers Chicken Diet Supplementation with Natural and Acidified Humic Substances on Quality of Produced Breast Meat},

author = {Marek Hudák and Boris Semjon and Dana Marcinčáková and Lukáš Bujňák and Pavel Naď and Beáta Koréneková and Jozef Nagy and Martin Bartkovský and Slavomír Marcinčák},

url = {https://www.mdpi.com/2076-2615/11/4/1087/htm https://www.mdpi.com/2076-2615/11/4/1087},

doi = {10.3390/ANI11041087},

issn = {20762615},

year  = {2021},

date = {2021-01-01},

journal = {Animals 2021, Vol. 11, Page 1087},

volume = {11},

issue = {4},

pages = {1087},

publisher = {Multidisciplinary Digital Publishing Institute},

abstract = {This study was conducted to examine the effect of two humic substances (HS) supplemented in broilers’ diet on the breast meat quality of broiler chickens. In this experiment, 120 pieces of one-day-old COBB500 broiler chickens were used. Broilers were divided into three groups, each containing 40 birds with three replications (13, 13, and 14 per one pen). Fattening lasted 38 days. The first experimental diet was supplemented with 0.7% of HS (HS0.7) and the second was enriched with 0.7% of acidified HS (HSA0.7). The control group of broilers (C) was fed a basal diet without the addition of any supplements. HS0.7 samples had the highest total protein content and the lowest content of fat (p < 0.01). The effects of broiler diet and storage had a significant impact on the pH of breast samples, p < 0.001 and p < 0.05, respectively. Supplementation of HS in broiler diet positively affected the lipid oxidation of breast meat samples, as indicated by the lower malondialdehyde content (p < 0.05). HSA0.7 samples had significantly better juiciness after the storage period (p < 0.001). The quality of meat produced with supplementation of HS in broilers’ feed can be considered as valuable in human nutrition due to improved protein and fat content with decreased lipid oxidation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Haus2021,

title = {The effect of humic acid in chronic deoxynivalenol intoxication},

author = {Martin Haus and Daniel Žatko and Janka Vašková and Ladislav Vaško},

url = {https://link.springer.com/10.1007/s11356-020-10581-x},

doi = {10.1007/s11356-020-10581-x},

issn = {0944-1344},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Science and Pollution Research},

volume = {28},

issue = {2},

pages = {1612-1618},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {The most common mycotoxin found in European foodstuffs, especially unprocessed grains, is deoxynivalenol (DON), which inhibits proteosynthesis and induces oxidative stress. The sorption properties of humic acids (HA) indicate a promising potential in the reduction of mycotoxin intoxication. In an experimental biomodel lasting 35 days, we investigated the effect of a dietary supplement containing HA on the antioxidant status in the liver, heart and kidney mitochondria and in the blood plasma of rats exposed to the stress factor of orally administered DON. DON was administered at doses of 100 and 200% above the maximum tolerable daily intake (1.0 μg/kg body weight/day). We evaluated the activities of the following enzymes: superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase and tripeptide glutathione. Administration of DON has been shown to induce oxidative stress, the intensity of which appears to be directly dependent on the concentration of mycotoxin. Supplementation with 1% HA (10 g/kg of feed) resulted in alleviation of this stress, in which the preventive effect of HA may have been implicated partly by affecting the adsorption of DON through the GIT and limiting its bioavailability. There were also signs that it can act by actively interfering with ROS inhibition to help achieve redox homeostasis. However, there is another aspect that deserves attention, namely, the metabolism of HA. The results presented in our work suggest that the mechanism of possible metabolism occurs predominantly in the liver, including an oxidative increase in polarity and subsequent conjugation reactions mediated by the GST-GSH system.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Dodatki funkcjonalne, takie jak sól, kwasy huminowe i mikroelementy – jako modulatory wydajności i dobrego zdrowia  wysokowydajnych krów mlecznych},

author = {Agata Szewczyk and Małgorzata Kuszta},

issn = {1731-8068},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Wiadomości Zootechniczne},

volume = {LIX},

issue = {4},

pages = {16-24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Humic Acid Mitigates the Negative Effects of High Rates of Biochar Application on Microbial Activity},

author = {Jiří Holátko and Tereza Hammerschmiedt and Rahul Datta and Tivadar Baltazar and Antonin Kintl and Oldřich Latal and Václav Pecina and Petr Sarec and Petr Novák and Ludmila Baláková and Subhan Danish and Muhammad Zafar-ul-Hye and Shah Fahad and Martin Brtnický

},

doi = {10.3390/su12229524},

year  = {2020},

date = {2020-11-01},

urldate = {2020-11-01},

journal = {Sustainability},

volume = {12},

issue = {9524},

pages = {9524},

abstract = {Biochar and a commercial humic acid-rich product, Humac (modified leonardite), represent soil amendments with the broad and beneficial effects on various soil properties. Their combination has been scarcely tested so far, although the positive impact of their interaction might be desirable. Materials and Methods: The dehydrogenase activity (DHA), microbial biomass carbon (C mic), soil respiration (basal and substrate-induced), enzyme activities, total carbon (C tot), and both shoot and root biomass yield were measured and compared in the short-term pot experiment with the lettuce seedlings. The following treatments were tested: the unamended soil (control), the Humac-amended soil (0.8 g·kg −1), the biochar-amended soil (low biochar 32 g·kg −1 , high biochar 80 g·kg −1), and the soil-amended with biochar + Humac. Results: The effect of both amendments on the soil pH was insignificant. The highest average values of C tot and C mic were detected in high biochar treatment and the highest average values of basal and substrate-induced respiration (glucose, glucosamine, alanine) were detected in the low biochar treatment. The phosphatase activity and fresh and dry lettuce aboveground biomass were the highest in the low biochar + Humac treatment. Conclusions: Even though the combination of both biochar + Humac decreased the microbial activities in the amended soil (C mic , DHA, enzymes, substrate-induced respiration) at the low biochar dose, they mitigated the detrimental effect of the high biochar dose on respiration (all the types) and the enzyme (phosphatase, arylsulphatase) activities. In contrast to the previously published research in this issue, the effects could not be attributed to the change of the soil pH.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of supplemental humic acid with urea as a nonprotein dietary nitrogen source on rumen variables in sheep},

author = {Lukáš Bujňák and Andrej Marcin and Pavel Naď and Tomáš Mihok},

doi = {SZIE.AWETH.2020.1.001},

year  = {2020},

date = {2020-04-04},

journal = {AWETH},

volume = {16},

issue = {1},

abstract = {The aim of this study was to investigate the effects of humic substances (HS) combined with urea as feed additives on the rumen fermentation indicators (pH, concentration of volatile fatty acids VFAs, ammonia concentration, protozoal population) in rumen fluid of 12 female crossbred merino sheep (n = 6 in test and control group). The daily ration for both groups consisted of 1.25 kg grass hay and 0.25 kg cereal grain mixture and 10 g urea. The humic substances were applied at a dose of 20 g/day per animal of the test group orally drenched with a probe directly into rumen (day 1 – 3) at the morning feeding or mixed into feed (day 4 – 18). Rumen samples were taken on day 0, 3 and 18 at 3, 6 and 9 h post morning feeding. The results revealed that HS increased ammonia concentration and protozoal cells of Entodinium spp. by 243.2 and of Diplodinium spp. by 5.1 (x103 /ml) on day 3, while the total VFAs concentration; acetate and propionate proportions as well as pH values of rumen fluid and the acetate : propionate ratio were not changed. Ammonia was higher (P≤0.05) in the test group by 7.9 at 6 h and by 5.6 mg/100ml at 9 h on day 3. Results suggest humic substances addition with urea as a non-protein dietary nitrogen source may improve nitrogen retention in the rumen, but there was no impact on VFAs production. The feed intake of HS with urea had significantly positive effects on the protozoal population in rumen fluid.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The effect of humic substances on gut microbiota and immune response of broilers},

author = {Dagmar Mudroňová and Viera Karaffová and T. Pešulová and Jana Koščová and I. Cingeľová Maruščáková and Martin Bartkovský and Daniela Marcinčáková and Z. Ševčíková and Slavomír Marcinčák},

url = {https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1707780},

doi = {10.1080/09540105.2019.1707780},

issn = {0954-0105},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-01},

journal = {Food and Agricultural Immunology},

volume = {31},

issue = {1},

pages = {137-149},

publisher = {Taylor and Francis Ltd.},

abstract = {The work focused on the study of the immunomodulatory and gut-protecting effect of humic substances (HS) in broilers. The diet of experimental chicks was enriched with 0.8% of HS. We noted that HS had a stimulatory effect on the phagocytic activity and the engulfing capacity of phagocytes, however, the level of oxidative burst of phagocytes was not affected. We observed a significant increase of CD4+: CD8+ lymphocyte ratio, an indicator of immune stimulation. HS did not influence the IgA gene expression. In contrast, we observed a significant increase in the expression of MUC-2 (intestinal mucin 2) gene, and a decrease in the expression of IGF-2 (insulin-like growth factor 2) and also AvBD2 (avian beta defensin 2) genes. A decreased Enterobacteriaceae counts in the gut of experimental animals showed a positive effect on intestinal microbiota. We confirmed a gut-protecting and an immunostimulatory effect of HS in broiler chickens.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Zigo2020,

title = {Impact of humic acid as an organic additive on the milk parameters and occurrence of mastitis in dairy cows},

author = {František Zigo and Milan Vasiľ and Zuzana Farkašová and Silvia Ondrašovičová and Martina Zigová and Jana Maľová and Jana Výrostková and Jolanta Bujok and Ewa Pecka-Kiełb},

url = {http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1340},

doi = {10.5219/1340},

issn = {13370960},

year  = {2020},

date = {2020-01-01},

journal = {Potravinarstvo Slovak Journal of Food Sciences},

volume = {14},

pages = {358-364},

publisher = {HACCP Consulting},

abstract = {Given growing concerns about the use of antibiotics in the treatment of animals, identifying organic alternatives as feed additives to improve animal health and the development of immune responses has become of interest in dairy farming. Humic acids (HA) seem to be a suitable alternative with a favorable impact on the health and production parameters of animals. This study aimed to determine the effects of an HA supplemented diet on milk parameters as well as the effects on somatic cell count (SCC) and the occurrence of mastitis in dairy cows during the peripartum period. Twenty dairy cows in the last stage of pregnancy were selected from a herd of 140 cows. The selected cows were randomly divided into two groups: control (C) and experimental (E). The two groups were fed the same feed mixture and group E was additionally supplemented with HA at a total dose of 100 g per cow per day during the last 50 days of pregnancy. The milk parameters (dry matter, lactose, fat, crude protein, casein and milk urea) and SCC of every cow, and the presence of mastitis, were checked on days 10 and 30 during the first month of lactation. The results of the study show that dietary supplementation with HA significantly reduced the milk urea (MU) content and SCC on the 10th day after calving but did not affect the other milk compositions. In addition to the decreased MU and SCC, the number of positive quarters detected by the California Mastitis Test was reduced by 20.0% and the occurrence of mastitis caused by coagulase-negative staphylococci (CNS). Based on the obtained results we can conclude that the addition of HA stabilizes the nutrient digestion, as was confirmed by a reduced MU content in the supplemented group. Their indirect beneficial effects improved the development of immune responses, resulting in decreased SCC and the occurrence of mastitis caused by CNS.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Semjon2020,

title = {Multiple factorial analysis of physicochemical and organoleptic properties of breast and thigh meat of broilers fed a diet supplemented with humic substances},

author = {Boris Semjon and Dana Marcinčáková and Beáta Koréneková and Martin Bartkovský and Jozef Nagy and Peter Turek and Slavomír Marcinčák},

doi = {10.1016/j.psj.2019.11.012},

issn = {15253171},

year  = {2020},

date = {2020-01-01},

journal = {Poultry Science},

volume = {99},

issue = {3},

pages = {1750-1760},

publisher = {Elsevier Inc.},

abstract = {Multiple factor analysis was used for the examination of meat quality of broilers, the diet of which was supplemented with 0.8 and 1.0% addition of humic substances (HS). One hundred fifty COBB 500 one-day-old male broilers chicks were randomly divided into 3 different groups: one control and 2 experimental (n = 50), and they were fattened for 35 D. Subsequently, the meat quality was analyzed and defined by physicochemical and sensory quality, supplemented with analysis by instrumental methods. We observed changes in dry matter, fat, water, and protein content in experimental samples of breast and thigh meat (P < 0.001). In both experimental groups, the concentration of phosphates and pH decreased in breast meat (P < 0.001) and in thigh meat (P < 0.05). The smell of experimental chicken breast meat samples after cooking was evaluated by a sensory panel, which scored a higher point score than that of the control group (P < 0.05). Sensory evaluation of taste indicated a positive response with respect to the perception of meat quality in relation to a greater supplementation of HS in the diet. Thigh meat samples showed a variable extent of water loss after cooking, but lower values of water loss were generally obtained from thigh meat samples of poultry fed with higher HS supplementation, than in chicken breast meat samples. Significant differences in evaluated variables between both experimental groups were not observed. The color of breast meat samples changed, when considering the variables of lightness and redness, with the addition of 1% HS (P < 0.05). The main advantage of the breast meat of broilers fed a diet supplemented with HS was observed in the final meat quality, which was positively affected by increased protein and decreased fat content. Because of its nutritional composition, it can be considered to be rather a valuable type of meat in human nutrition than ordinarily.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of humic acids on lead poisoning in bones and on a subcellular level in mitochondria},

author = {Janka Vašková and Ladislav Vaško and Pavol Mudroň and Martin Haus and Daniel Žatko and Klára Krempaská and Marek Stupák},

doi = {10.1007/s11356-020-10075-w},

issn = {16147499},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

volume = {27},

issue = {32},

pages = {40679-40689},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Humic acids (HA) are natural substances which exhibit a remarkable spectrum of health benefits, such as their role in chelation. This study aims to supplement the current knowledge on the chelating effects of HA in chronic lead intoxication in rat femurs and in liver, heart and kidney mitochondria in an experiment lasting 10 weeks. Lead acetate trihydrate was administered to rats for 5 weeks at a daily dose of 155.5 mg/kg body weight. At the same time, rats were given three concentrations of HA, with their effect measured over the following 5 weeks. Increased Pb concentrations were detected in the femur after the first week, while HA-administered groups showed a tendency towards inhibiting the increase in Pb deposition. After 5 weeks, Pb concentrations dropped significantly in the HA groups. At the same time, however, other elements were redistributed, with a decrease in Se and Zn being particularly noteworthy. While an increase in Pb concentrations was found after 5 weeks of Pb administration, a concentration of 1% HA resulted in the least significant increase in Pb as well as an increase/decrease in Se/Cu, respectively. In mitochondria, an increase in Pb content was detected after the first and fifth weeks with concomitant redistribution of other elements. At the end of the experiment, again in the 1% HA group, Pb concentrations remained higher only in the liver with the other elements sufficiently normalized, indicating this concentration to be useful in the treatment of Pb intoxication.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effects of Humic Acids in Chronic Lead Poisoning},

author = {Janka Vašková and Klára Krempaská and Daniel Žatko and Pavol Mudroň and Gabriela Glinská and Ladislav Vaško},

url = {http://link.springer.com/10.1007/s12011-018-1375-1},

doi = {10.1007/s12011-018-1375-1},

issn = {0163-4984},

year  = {2019},

date = {2019-01-01},

journal = {Biological Trace Element Research},

volume = {187},

issue = {1},

pages = {230-242},

publisher = {Humana Press Inc.},

abstract = {Chronic exposure to lead causes disruption to energy production mechanisms and tissue damage, in particular through its binding to thiol groups and competition for zinc binding sites. We investigated the possibility of preventing the consequences of chronic lead poisoning by administration of three different doses of humic acids (HAs) into feed with the aim of establishing an effective HA dose. During the 10-week experiment, a sub-lethal dose of lead acetate was given to rats during the first 5 weeks, with continuous administration of HA over 10 weeks. Measurements were taken to determine the content of the metals Pb, Mn, Cu, Fe and Zn; the metalloid Se; and selected antioxidant markers in the heart, liver, kidney and plasma after the first, fifth and tenth weeks of experiment. The administration of lead and HAs clearly affects the redistribution of the elements and the activity of the antioxidant enzymes. This fact was particularly highlighted in the lead-only group as, within the experiment, significantly higher Pb concentrations were found only in the plasma of this group. However, in the group with 1% HA administered with lead, we observed a rise in Zn concentrations in the organs and the deposition of Fe into the liver. Decreased glutathione reductase activity in the plasma and balanced reduced glutathione concentrations indicated sufficient efficiency of redox reactions. SOD activities were among those affected most strongly, with only the 1% HA group showing no effect on heavy metal redistribution as a result of HA administration.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The effect of dietary humic substances on the fattening performance, carcass yield, blood biochemistry parameters and bone mineral profile of broiler chickens},

author = {Iveta Jaďuttová and Dana Marcinčáková and Martin Bartkovský and Boris Semjon and Michaela Harčárová and Alena Nagyová and Peter Váczi and Slavomír Marcinčák},

doi = {10.2754/avb201988030307},

issn = {18017576},

year  = {2019},

date = {2019-01-01},

journal = {Acta Veterinaria Brno},

volume = {88},

issue = {3},

pages = {307-313},

publisher = {University of Veterinary and Pharmaceutical Sciences},

abstract = {Humic substances are currently being considered as a new feed component in replacement of antibiotic growth stimulators to improve growth and health in animals. The aim of our work was to evaluate the effect of the addition of humic substances to the feed at concentrations of 0.8% and 1.0% on the growth parameters, carcass yield, selected blood enzymes, minerals, and the bone quality in broiler chickens. One hundred fifty COBB 500 one-day-old male broiler chicks were randomly divided into one control and two experimental groups (n = 50) with three replications (16, 16 and 17 per pen), and fattened for 35 days. The broilers of the experimental groups (H0.8 and H1.0) were fed commercial feed mixtures with added humic substances (at 8 g·kg-1 and 10 g·kg-1). The control group was fed a diet without the addition of humic substances. A slight increase in the final body weight and feed conversion ratio was noted in the experimental groups (P > 0.05). Significantly higher yields of breast and thigh meat (P < 0.05) were recorded for the experimental group H1.0. The blood cholesterol level was lower for both experimental groups. A lower concentration of alkaline-phosphatase was observed in group H1.0. A decrease in aspartate aminotransferase and calcium was recorded for group H0.8. A significantly higher content of calcium and lower content of phosphorus was found in the bones of experimental animals. The 1% addition of humic substances had a positive impact on growth parameters (P > 0.05), improved selected blood indices and increased the calcium content in the bones of broilers (P < 0.05).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The amino acid profile after addition of humic acids and phytobiotics into diet of broiler chicken},

author = {Peter Haščík and Adriana Pavelková and Jana Tkáčová and Juraj Čuboň and Marek Bobko and Miroslava Kačániová and Henrieta Arpášová and Matej Čech},

doi = {10.5219/1237},

issn = {13370960},

year  = {2019},

date = {2019-01-01},

journal = {Potravinarstvo Slovak Journal of Food Sciences},

volume = {13},

issue = {1},

pages = {884-890},

publisher = {HACCP Consulting},

abstract = {The aim of the study was analysed the effect of humic acids separately and humic acids in combination with phytobiotic as garlic and oregano powder on amino acid (AA) profile of the most valuable parts of Ross 308 chicken. A total of 200 pcs Ross 308 broiler chickens of mixed sex were randomly divided into 4 groups (n=50): control group (C) without supplementation, experiment group E1 (2% humic acids), E2 (80% humic acids and 20% garlic powder) and E3 (90% humic acids and 10% oregano powder). Fattening period lasted for 42 days and all groups were kept under the same conditions. After slaughter, the AA profiles of breast and thigh samples were determined. In comparison with control group, 6 out of 10 AA was significantly affected (p ≤0.05) by used dietary supplementation-Met, Cys and His in thigh and Leu, Phe, His and Arg in breast muscle. AA composition of breast muscle was positively affected mainly by humic acids and 10% oregano powder supplementation (E3), while thigh muscle by humic acids and 20% garlic powder (E2). The highest obtained AA in breast muscle was Leu (2.02 g.100 g-1) in E3 group and thigh muscle His (1.15 g.100 g-1) in E2 group (p ≤0.05). In conclusion, humic acids and 10% oregano powder supplementation (E3) elicited to the best AA profile of chicken breast muscle but also the worst AA profile in thigh muscle so the effect of such a supplementation is disputable. On the other hand, humic acids and 20% garlic powder supplementation resulted into slight increase of AA in both breast and thigh muscle (E2).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Chemical composition of chicken meat after application of humic acid and probiotic Lactobacillus fermentum},

author = {Peter Haščík and Henrieta Arpášová and Adriana Pavelková and Marek Bobko and Juraj Čuboň and Ondrej Bučko},

doi = {10.5219/943},

issn = {1337-0960},

year  = {2018},

date = {2018-10-29},

journal = {Potravinarstvo Slovak Journal of Food Sciences},

volume = {12},

issue = {1},

pages = {694-700},

abstract = {The  aim  of  the present study  was analysed  and  evaluatedchemical parameters of  chicken breast  and  thigh  muscles after addition of humic acids and probiotic into diet for broiler chicken.A total of 200pcsRoss 308 broiler chickens were divided into 4 groups (n=50). The control group of chickens was fed with complete feed mixtures without any additives. Chickensin experiment groupswere  fed  a  diet  containing:  P1(1%  of  humic  acid),  P2 (1%  of  humic  acid  andprobiotic  supplementLactobacillus fermentum) andP3 were fed with complete feed mixture containingcombination of starterfeed mixture (1. –21.  day) with  coccidiostaticum  Diclazuril  and  growth  feed  mixture (21. –35.  day) containing  Salinomycinum  sodium. Besides, the groups were kept under the same conditions. Fattening period lasted for 42 days. Chicken meat was analyzed for content of water, crude protein, fat and cholesterol.Based on the results, we can state that the application of humic acidsorthe combination of HumacNaturwithprobiotic did not affect the chemical composition of the breast muscle.In the breast muscle,  the  protein  content  in  the experimental  group P3 with  the  coccidiostat  (22.98  g.100  g-1)  was  reduced  (p≤0.05) compared to controlgroup(23.42 g.100 g-1). In the case of thigh muscle was significantly higher content offat and cholesterol (p≤0.05) in chickens feeding with addition of Humac Natur(fat –9.08 g.100g-1; cholesterol –0.86 mg.100g-1) and similar results were recordedin experimental group with combination of HumacNatur and probiotic (fat–9.15 g.100g-1; cholesterol –0.86 mg.100g-1) compared to control group (fat –7.15g.100g-1; cholesterol –0.70 mg.100g-1). From a  general  point of view, we can recommend the application of Humac Natur, respectivelycombination Humac Natur with probiotics in feeding of broiler chickens Ross 308.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The effect of single and combined use of probiotic and humate on fattening performance, carcass characteristics and internal organs of broiler chickens},

author = {Cyril Hrnčár and Nedeljka Nikolova and Jozef Bujko},

issn = {1857 – 6907},

year  = {2018},

date = {2018-08-31},

journal = {Macedonian Journal of Animal Science},

volume = {8},

number = {2},

pages = {81-87},

abstract = {The aim of this study was to evaluate the both single and combined effects of probiotic and humate on performance and carcass characteristics of broiler chickens. Totally 200 one-day old broiler chickens Ross 308 were divided into one control group and three experimental groups. The control group (n = 50) was without probiotic and humate, experimental groups with 3.7 ml on 1 litre of drinking water (Group P, n = 50), 50 g per 10 kg of feed (Group H, n = 50) and 3.7 ml probiotic per litre of drinking water + 50 g humate per 10 kg of feed (Group P + H, n = 50), respectively. Application of probiotic and humate both single and combined statistically significant increased (p < 0.05) final body weight and reduced feed consumption (p < 0.05). From carcass characteristics, application of probiotic and probiotic and humate combination statistically significant decreased (p < 0.05) abdominal fat weight. Supplementation of single and combined use of probiotic and humate no significantly affected (p > 0.05) breast and thighs percentages and carcass yield. The caecum, small intestine and large intestine proportions significantly (p < 0.05) increased in chickens fed diets containing probiotic compared to control. For neck, crop, heart, liver, proventriculus, gizzard, pancreas and kidneys proportions among control and experimental groups we recorded no statistical differences (p > 0.05).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}